Milad Alizadeh

TL;DR: The current manuscript reviews the advances in Cannabis biotechnology, including molecular markers, microRNA, omics-based methods, and functional genes related to the terpene and cannabinoid biosynthesis as well as fiber quality.

TL;DR: This is the first report of an effective in vitro organogenesis protocol for F. religiosa by indirect shoot organogenesis through axenic seedling derived petiole explants, which can be efficiently employed for conservation of this important medicinal plant species as well as the utilization of active biomolecules.

TL;DR: In this article, a review of in vitro culture and genetic engineering methods in cannabis along with other promising techniques such as morphogenic genes, new computational approaches, clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR/Cas9-equipped Agrobacterium-mediated genome editing, and hairy root culture, that can help improve gene transformation and plant regeneration, as well as enhance secondary metabolite production, have been highlighted and discussed.

TL;DR: Generally, the developed hybrid model in this study (ensemble model-FOA) can be employed as an accurate and reliable approach in future genetic engineering and genome editing studies.

TL;DR: In this article, the effect of different concentrations of sodium nitroprusside on the appearance of callus browning from leaf explants was investigated and the results indicated that callus Browning was significantly reduced by supplementation of sodium n-propusside to the MS medium and supplemented with 2,4-dichlorophenoxyacetic acid and 0.22-μm of 6-benzyl amino purine.