Showing papers by "Mohamed Al-Rubeai published in 1990"
TL;DR: The kinetics of monoclonal antibody synthesis and secretion have been studied in synchronous and asynchronous mouse hybridoma cell cultures to show consistent with the idea that slow growth and higher death rates increase QA and that Ig secretion is probably subject to complex intracellular control.
135 citations
TL;DR: Electron microscopy of mouse hybridoma cell lines shows that the major difference between non, low and high producer cell lines is the amount of endoplasmic reticulum.
Abstract: Electron microscopy of mouse hybridoma cell lines shows that the major difference between non, low and high producer cell lines is the amount of endoplasmic reticulum. Vesicular-tubular or cavernous structures of endoplasmic reticulum, which can survive long after cell death, are particularly abundant in producer cell lines. Immunogold labelling with anti-mouse IgG reveals that antibodies are predominantly located in these structures. The cell membrane undergoes structural changes during the late stages of batch culture with the disappearance of microvilli and the appearance of blebs and deep indentations. Necrosis disrupts the cytoplasmic structures and the nucleus is last to degrade.
63 citations
TL;DR: The responses of hybridoma growth and metabolism to hydrodynamic forces and changes in culture temperature have been examined and DNA synthesis is inhibited under conditions of intense hydrod dynamic stress; however, cellular metabolic activity is increased perhaps to feed repair mechanisms.
Abstract: The responses of hybridoma growth and metabolism to hydrodynamic forces and changes in culture temperature have been examined. DNA synthesis is inhibited under conditions of intense hydrodynamic stress; however, cellular metabolic activity is increased perhaps to feed repair mechanisms. Sub-optimal temperature and nutrient deprivation increase cellular susceptibility to hydrodynamic stress, possibly through the inhibition of repair mechanisms. The reduced cell number in conditions of high agitation may be due at least in part to growth inhibition.
46 citations