Monique Nicoloso

TL;DR: It is shown that altering the sequence of the snoRNA is sufficient to change the specificity of methylation and endogenous non-ribosomal RNA can thus be targeted, possibly providing a highly selective tool for the alteration of gene expression at the post-transcriptional level.

TL;DR: Nine additional members of the snoRNAs identified are intron-encoded, contain the characteristic box C and box D motifs and exhibit long complementarities to conserved sequences in mature rRNAs, supporting the conclusion that the duplex structure provides the 2'-O-methyltransferase with the appropriate site-specificity on the substrate.

TL;DR: Identification of the yeast Saccharomyces cerevisiae U24 gene directly confirms the outstanding conservation of the complementarity to 28S rRNA during evolution, suggesting a key role of U24 pairing to pre-rRNA during ribosome biogenesis, possible in the control of pre- rRNA folding.

TL;DR: While the DMS probing of deproteinized rRNA confirms a number of helical stems predicted by phylogenetic comparisons, it is remarkable that a few additional base-pairings appear to require the presence of the bound ribosomal subunits to be stabilized.

TL;DR: U20 RNA contains an extended region (21 nucleotides) of perfect complementarity with a phylogenetically conserved sequence in 18S rRNA, suggesting that U20 RNA may be involved in the formation of the small ribosomal subunit like nucleolin, the product of its host gene.