Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction

http://academic.oup.com/milmed/article-pdf/146/7/506/24126715/milmed-146-7-506.pdf

Goodman and Gilman's The Pharmacological Basis of Therapeutics

Treatment of Asthma with Drugs Modifying the Leukotriene Pathway

The mechanism of chemotherapy-induced acceleration of ovarian aging is not fully understood. We used doxorubicin, a widely used cancer chemotherapeutic, in a variety of in vivo xenograft, and in vitro models to investigate the impact of chemotherapy-induced aging on the human ovary. Doxorubicin caused massive double-strand-DNA-breaks in primordial follicles, oocytes, and granulosa cells in a dose dependent fashion as revealed by accumulating γH2AX foci. This damage was associated with apoptotic oocyte death and resulted in the activation of ATM. It appeared that the repair response enabled a minor proportion of oocytes (34.7%) and granulosa cells (12.1%) to survive while the majority succumbed to apoptotic death. Paradoxically, inhibition of ATM by KU-55933 resulted in improved survival, probably via prevention of downstream activation of TAp63α. Furthermore, doxorubicin caused vascular and stromal damage in the human ovary, which might impair ovarian function both pre- and post-menopausally. Chemotherapy-induced premature ovarian aging appears to result from a complex process involving both the germ- and non-germ cell components of the ovary. These effects may have clinical implications in aging both for premenopausal and postmenopausal cancer survivors.

https://s3-us-west-1.amazonaws.com/paperchase-aging/pdf/kKneXNXRuaNafkHKG.pdf

Mechanisms of chemotherapy-induced human ovarian aging: double strand DNA breaks and microvascular compromise

Although most studies have failed to demonstrate an elevated risk of major birth defects in children conceived by intracytoplasmic sperm injection (ICSI) or standard in vitro fertilization (IVF), most past work is methodologically problematic and definitions of major defects may differ from those applied to naturally conceived infants. This study examined the prevalence of major birth defects diagnosed within 12 months after birth in infants conceived either naturally or using ICSI or IVF. All births took place in Western Australia in the years 1993 to 1997. Data from a reproductive technology registry identified all pregnancies of at least 20 weeks' gestation. The study population included 301 infants conceived by ICSI; 837 conceived by standard IVF; and 4000 conceived naturally. Infants conceived with assisted methods were likelier than those conceived naturally to be delivered by cesarean section, to be born before term, and to have low birth weight. Major birth defects were identified by age 1 year in 8.6% of infants conceived with ICSI, 9.0% of the IVF group, and 4.2% of naturally conceived infants. In all, infants conceived using assisted reproductive technology were more than twice as likely as those conceived naturally to have a major defect. The difference remained significant when only singleton infants, or only singletons born at term, were analyzed, and after adjusting for maternal age and parity and infant gender. Approximately two thirds of major defects were diagnosed in the first week of life, and more than 90% by age 6 months. Excluding defects that may have been diagnosed early because of close surveillance, the odds ratio for a major defect was 1.8 in the ICSI group and 2.2 in the IVF group. Major defects remained about twice as frequent in these groups as with natural conception after excluding infants known to have inherited conditions and those with metabolic disorders. Musculoskeletal and chromosomal defects were significantly more prevalent with assisted reproductive technology. The IVF group (but not ICSI infants) had significantly more cardiovascular, urogenital, and other defects. Multiple major defects were significantly more frequent in the IVF and ICSI groups. Minor birth defects were diagnosed by age 12 months in less than 1% of all groups. These findings support the possibility of an excessive risk of major birth defects in infants born with the benefit of assisted reproductive technology. Nevertheless caution is in order when establishing associations with particular birth defects.

The risk of major birth defects after intracytoplasmic sperm injection and in vitro fertilization

We investigated the potential protective effects of montelukast (MLK) on cecal ligation and puncture (CLP)–induced tissue injury in vital organs — liver, heart, kidneys, and especially lungs — through inhibition of the proinflammatory cytokine response and the generation of reactive oxygen species (ROS) in rats. The rat groups were (1) a 10-mg/kg MLK-treated CLP group; (2) a 20-mg/kg MLK-treated CLP group; (3) a 20-mg/kg MLKtreated, sham-operated group; (4) a CLP control group; and (5) a sham-operated control group. MLK treatment significantly decreased proinflammatory (tumor necrosis factor-α, interleukin-6) cytokine levels following CLP. The lipid peroxide level increased in the lung, heart, liver, and kidney tissues after CLP-induced sepsis, and myeloperoxidase activity increased in the lung, heart, and liver tissues. MLK attenuated this elevation in all tissues except the kidney, dose dependently. The glutathione levels and superoxide dismutase activity were significantly increased in the lung, liver, and kidney tissues after MLK treatment. MLK treatment after CLP also potentially reduced mortality. The lung and kidney tissues were the most protected by MLK under sepsis conditions. We can suggest that MLK reverses the systemic inflammatory reaction to polymicrobial sepsis and thereby reduces multiple organ failure.

https://downloads.hindawi.com/journals/tswj/2011/657560.pdf

The effects of montelukast on antioxidant enzymes and proinflammatory cytokines on the heart, liver, lungs, and kidneys in a rat model of cecal ligation and puncture-induced sepsis.

Hepatic laceration because of malpositioning of the umbilical vein catheter: case report and literature review.

Growth hormone reduces tissue damage in rat ovaries subjected to torsion and detorsion: biochemical and histopathologic evaluation.

http://www.if-pan.krakow.pl/pjp/pdf/2011/2_518.pdf

Antiulcerative effect of dexmedetomidine on indomethacin-induced gastric ulcer in rats

Purpose
The aim of this study was to compare the complication rates of the single flap and double flaps versus flapless procedure in the tubularized incised plate urethroplasty.

Murat Yigiter

Papers

The effects of montelukast on antioxidant enzymes and proinflammatory cytokines on the heart, liver, lungs, and kidneys in a rat model of cecal ligation and puncture-induced sepsis.

Hepatic laceration because of malpositioning of the umbilical vein catheter: case report and literature review.

Growth hormone reduces tissue damage in rat ovaries subjected to torsion and detorsion: biochemical and histopathologic evaluation.

Antiulcerative effect of dexmedetomidine on indomethacin-induced gastric ulcer in rats

A comparative study to evaluate the effect of double dartos flaps in primary hypospadias repair: no fistula anymore