Narayanan Kasthuri

TL;DR: It is shown that filopodia-like dendritic protrusions, extending and retracting over hours, are abundant in young animals but virtually absent from the adult, providing a potential structural basis for long-term information storage.

TL;DR: In this paper, the authors describe automated technologies to probe the structure of neural tissue at nanometer resolution and use them to generate a saturated reconstruction of a sub-volume of mouse neocortex in which all cellular objects (axons, dendrites, and glia) and many subcellular components (synapses, synaptic vesicles, spines, spine apparati, postsynaptic densities, and mitochondria) are rendered and itemized in a database.

TL;DR: High-throughput electron microscopy reconstructions of single axons of pyramidal neurons in the mouse neocortex are traced and it is found that individual neurons have distinct longitudinal distribution of myelin.

TL;DR: In the CA1 layer of the hippocampal network, a compound oscillatory input may be segregated into distinct frequency components which are processed locally by distinct types of neurones.

TL;DR: This technique uses osmium impregnation (OTO) to make the samples conductive while heavily staining membranes for segmentation studies to produce clean, highly contrasted TEM and scanning electron microscopy (SEM) samples of insect, fish and mammalian nervous systems.