Juan Carlos Tapia

TL;DR: In this paper, the authors describe automated technologies to probe the structure of neural tissue at nanometer resolution and use them to generate a saturated reconstruction of a sub-volume of mouse neocortex in which all cellular objects (axons, dendrites, and glia) and many subcellular components (synapses, synaptic vesicles, spines, spine apparati, postsynaptic densities, and mitochondria) are rendered and itemized in a database.

TL;DR: This technique uses osmium impregnation (OTO) to make the samples conductive while heavily staining membranes for segmentation studies to produce clean, highly contrasted TEM and scanning electron microscopy (SEM) samples of insect, fish and mammalian nervous systems.

TL;DR: Evidence is provided for a direct role of gamma-Pcdhs in synaptic development and genetic tools for elucidating their contribution to synaptic specificity are established.

TL;DR: Analysis of skeletal neuromuscular junctions in mice reveals novel structural, regional and molecular parallels between aging and ALS and finds that muscles respond in one of three ways to aging.

TL;DR: It is demonstrated that CHT is an essential and regulated presynaptic component of cholinergic signaling and indicates that it warrants consideration as a candidate gene for disorders characterized byCholinergic hypofunction.