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Natasha Karassina
Researcher at Promega
Publications - 21
Citations - 2229
Natasha Karassina is an academic researcher from Promega. The author has contributed to research in topics: Metabolic pathway & Jurkat cells. The author has an hindex of 10, co-authored 18 publications receiving 1842 citations.
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Journal ArticleDOI
HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis
Georgyi V. Los,Lance P. Encell,Mark McDougall,Danette Hartzell,Natasha Karassina,Chad Zimprich,Monika G. Wood,Randy Learish,Rachel Friedman Ohana,Marjeta Urh,Daniel J. Simpson,Jacqui Méndez,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Ji Zhu,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +19 more
TL;DR: The utility of this modular protein tagging system for cellular imaging and protein immobilization is demonstrated by analyzing multiple molecular processes associated with NF-kappaB-mediated cellular physiology, including imaging of subcellular protein translocation and capture of protein--protein and protein--DNA complexes.
Journal ArticleDOI
Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.
Lance P. Encell,Rachel Friedman Ohana,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Monika G. Wood,Georgyi V. Los,Mark McDougall,Chad Zimprich,Natasha Karassina,Randall D. Learish,Robin Hurst,James Robert Hartnett,Sarah Wheeler,Pete Stecha,Jami English,Kate Zhao,Jacqui Méndez,Hélène A Benink,Nancy Murphy,Danette L. Daniels,Michael R. Slater,Marjeta Urh,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +26 more
TL;DR: HaloTag® (HT7), a genetic fusion tag based on a modified haloalkane dehalogenase designed and engineered to overcome the limitation of affinity tags by forming a high affinity, covalent attachment to a binding ligand, is described.
SUPPLEMENTARY MATERIAL Development of a Dehalogenase-Based Protein Fusion Tag Capable of Rapid, Selective and Covalent Attachment to Customizable Ligands
Lance P. Encell,Rachel Friedman Ohana,Kris Zimmerman,Paul Otto,Monika G. Wood,Georgyi V. Los,Mark McDougall,Chad Zimprich,Natasha Karassina,Randall D. Learish,Robin Hurst,James Robert Hartnett,Sarah Wheeler,Pete Stecha,Jami English,Kate Zhao,Jacqui Méndez,Hélène A Benink,Nancy Murphy,Danette L. Daniels,Michael R. Slater,Marjeta Urh,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +25 more
Journal ArticleDOI
XAF1 Mediates Tumor Necrosis Factor-α-induced Apoptosis and X-linked Inhibitor of Apoptosis Cleavage by Acting through the Mitochondrial Pathway
Shawn L. Straszewski-Chavez,Irene Visintin,Natasha Karassina,Georgyi V. Los,Peter Liston,Ruth Halaban,Ahmed Fadiel,Gil Mor +7 more
TL;DR: It is demonstrated that XAF1 functions as an alternative pathway for TNF-α-induced apoptosis by translocating to the mitochondria and promoting XIAP inactivation, and it is shown that the overexpression ofXAF1 sensitized first trimester trophoblast cells to Fas-mediated apoptosis.
Journal ArticleDOI
In vivo stable tumor-specific painting in various colors using dehalogenase-based protein-tag fluorescent ligands.
Nobuyuki Kosaka,Mikako Ogawa,Peter L Choyke,Natasha Karassina,Cesear Corona,Mark McDougall,David T. Lynch,Clifford Hoyt,Richard M. Levenson,Georgyi V. Los,Hisataka Kobayashi +10 more
TL;DR: This tagging system proves versatile both for in vivo and in vitro imaging without requiring modification of the underlying cell line, and can overcome some of the limitations associated with the use of endogenous fluorescent proteins and exogenous targeted optical agents in current use.