R
Robin Hurst
Researcher at Promega
Publications - 26
Citations - 826
Robin Hurst is an academic researcher from Promega. The author has contributed to research in topics: Analyte & Protein purification. The author has an hindex of 12, co-authored 24 publications receiving 592 citations.
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Journal ArticleDOI
CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide
Marie K. Schwinn,Thomas Machleidt,Kris Zimmerman,Christopher T. Eggers,Andrew S. Dixon,Robin Hurst,Mary P. Hall,Lance P. Encell,Brock Binkowski,Keith V. Wood +9 more
TL;DR: The ability to efficiently tag endogenous proteins with a small luminescent peptide is demonstrated, allowing sensitive quantitation of the response dynamics in their regulated expression and covalent modifications.
Journal ArticleDOI
Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals
Yichi Su,Joel R. Walker,Yunhee Park,Thomas P. Smith,Lan Xiang Liu,Mary P. Hall,Louai Labanieh,Robin Hurst,David C. Wang,Lance P. Encell,Namdoo Kim,Namdoo Kim,Feijie Zhang,Mark A. Kay,Kerriann M. Casey,Robbie G. Majzner,Jennifer R. Cochran,Crystal L. Mackall,Thomas A. Kirkland,Michael Z. Lin +19 more
TL;DR: NanoLuc substrates with improved solubility and bioavailability, hydrofurimazine and fluorofurimazine, strongly enhance bioluminescence signals in vivo and enable bright dual-color biolUMinescent imaging with AkaLuc and AkaLumine.
Journal ArticleDOI
Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.
Lance P. Encell,Rachel Friedman Ohana,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Monika G. Wood,Georgyi V. Los,Mark McDougall,Chad Zimprich,Natasha Karassina,Randall D. Learish,Robin Hurst,James Robert Hartnett,Sarah Wheeler,Pete Stecha,Jami English,Kate Zhao,Jacqui Méndez,Hélène A Benink,Nancy Murphy,Danette L. Daniels,Michael R. Slater,Marjeta Urh,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +26 more
TL;DR: HaloTag® (HT7), a genetic fusion tag based on a modified haloalkane dehalogenase designed and engineered to overcome the limitation of affinity tags by forming a high affinity, covalent attachment to a binding ligand, is described.
SUPPLEMENTARY MATERIAL Development of a Dehalogenase-Based Protein Fusion Tag Capable of Rapid, Selective and Covalent Attachment to Customizable Ligands
Lance P. Encell,Rachel Friedman Ohana,Kris Zimmerman,Paul Otto,Monika G. Wood,Georgyi V. Los,Mark McDougall,Chad Zimprich,Natasha Karassina,Randall D. Learish,Robin Hurst,James Robert Hartnett,Sarah Wheeler,Pete Stecha,Jami English,Kate Zhao,Jacqui Méndez,Hélène A Benink,Nancy Murphy,Danette L. Daniels,Michael R. Slater,Marjeta Urh,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +25 more
Journal ArticleDOI
HaloTag-based purification of functional human kinases from mammalian cells.
Rachel Friedman Ohana,Robin Hurst,Jolanta Vidugiriene,Michael R. Slater,Keith V. Wood,Marjeta Urh +5 more
TL;DR: HaloTag fluorescent ligands enable fluorescent labeling of HaloTag fusion proteins, providing a convenient way to monitor expression, and thus facilitate the identification of optimal transient transfection conditions as well as the selection of high expression stable cell lines.