R
Rachel Friedman Ohana
Researcher at Promega
Publications - 31
Citations - 2758
Rachel Friedman Ohana is an academic researcher from Promega. The author has contributed to research in topics: Dehalogenase & Hydrolase. The author has an hindex of 12, co-authored 30 publications receiving 2213 citations.
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Journal ArticleDOI
HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis
Georgyi V. Los,Lance P. Encell,Mark McDougall,Danette Hartzell,Natasha Karassina,Chad Zimprich,Monika G. Wood,Randy Learish,Rachel Friedman Ohana,Marjeta Urh,Daniel J. Simpson,Jacqui Méndez,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Ji Zhu,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +19 more
TL;DR: The utility of this modular protein tagging system for cellular imaging and protein immobilization is demonstrated by analyzing multiple molecular processes associated with NF-kappaB-mediated cellular physiology, including imaging of subcellular protein translocation and capture of protein--protein and protein--DNA complexes.
Journal ArticleDOI
Target engagement and drug residence time can be observed in living cells with BRET.
Matthew B. Robers,Melanie Dart,Carolyn C. Woodroofe,Chad Zimprich,Thomas A. Kirkland,Thomas Machleidt,Kevin Kupcho,Sergiy Levin,James Robert Hartnett,Kristopher Zimmerman,Andrew L. Niles,Rachel Friedman Ohana,Danette L. Daniels,Michael R. Slater,Monika G. Wood,Mei Cong,Yi-Qiang Cheng,Keith V. Wood +17 more
TL;DR: A broadly applicable method using bioluminescence resonance energy transfer (BRET) to reveal the binding characteristics of a drug with selected targets within intact cells to demonstrate a novel application of BRET for assessing target engagement within the complex milieu of the intracellular environment.
Journal ArticleDOI
Quantitative, Wide-Spectrum Kinase Profiling in Live Cells for Assessing the Effect of Cellular ATP on Target Engagement
James D Vasta,Cesear Corona,Jennifer Wilkinson,Chad Zimprich,James Robert Hartnett,Morgan R. Ingold,Kristopher Zimmerman,Thomas Machleidt,Thomas A. Kirkland,Kristin G. Huwiler,Rachel Friedman Ohana,Michael R. Slater,Paul Otto,Mei Cong,Carrow I. Wells,Benedict-Tilman Berger,Thomas Hanke,Carina Glas,Carina Glas,Ke Ding,Ke Ding,David H. Drewry,Kilian Huber,Kilian Huber,Timothy M. Willson,Stefan Knapp,Susanne Müller,Poncho Meisenheimer,Frank Fan,Keith V. Wood,Matthew B. Robers +30 more
TL;DR: An energy transfer technique is reported that enabled the first broad-spectrum, equilibrium-based approach to quantitatively profile target occupancy and compound affinity in live cells and revealed improved target selectivity compared with biochemical measurements.
Journal ArticleDOI
HaloTag7: a genetically engineered tag that enhances bacterial expression of soluble proteins and improves protein purification.
Rachel Friedman Ohana,Lance P. Encell,Kate Zhao,Daniel J. Simpson,Michael R. Slater,Marjeta Urh,Keith V. Wood +6 more
TL;DR: A novel protein tag engineered to enhance expression and solubility of recombinant proteins and to provide efficient protein purification coupled with tag removal, designed to bind rapidly and covalently with a unique synthetic linker to achieve an essentially irreversible attachment.
Journal ArticleDOI
Development of a dehalogenase-based protein fusion tag capable of rapid, selective and covalent attachment to customizable ligands.
Lance P. Encell,Rachel Friedman Ohana,Kris Zimmerman,Paul Otto,Gediminas Vidugiris,Monika G. Wood,Georgyi V. Los,Mark McDougall,Chad Zimprich,Natasha Karassina,Randall D. Learish,Robin Hurst,James Robert Hartnett,Sarah Wheeler,Pete Stecha,Jami English,Kate Zhao,Jacqui Méndez,Hélène A Benink,Nancy Murphy,Danette L. Daniels,Michael R. Slater,Marjeta Urh,Aldis Darzins,Dieter Klaubert,Robert F. Bulleit,Keith V. Wood +26 more
TL;DR: HaloTag® (HT7), a genetic fusion tag based on a modified haloalkane dehalogenase designed and engineered to overcome the limitation of affinity tags by forming a high affinity, covalent attachment to a binding ligand, is described.