Natsuko Kakudo

TL;DR: Clinical platelet-rich plasma application for cell-based, soft-tissue engineering and wound healing is supported, as its activation was associated with the release of large amounts of PDGF-AB and TGF-&bgr;1 and its activation promoted the proliferation of human adipose–derived stem cells and human dermal fibroblasts.

TL;DR: FGF-2 significantly enhances the adipogenic differentiation of human ASCs, as induced by an adipogenic hormonal cocktail consisting of 3-isobutyl-1-methylxanthine (IBMX), dexamethasone, insulin, and indomethacin.

TL;DR: The composition of PRP produced by commercially available separation systems was characterized, and the mean PDGF-AB concentration of activated PRP was the highest from JP200, followed by the KYOCERA Medical PRP Kit, Magellan Autologous Platelet Separator System, MyCells, and GLO PRP.

TL;DR: Honeycomb collagen scaffold is a suitable scaffold for ASCs and will be useful as a three-dimensional bone tissue engineering scaffold in vitro and in vivo, according to histological views.

TL;DR: Adipose tissue-derived stem cells (ASCs) isolated from human subcutaneous adipose tissue were cultured in hypoxic or normoxic conditions and proliferation was significantly enhanced in the hypoxic culture and was inhibited by ERK and Akt inhibitors.