Nicholas J. Fuller

TL;DR: Clade-specific oligonucleotides for the marine Synechococcus genus were designed and their specificity was optimized and a predominance of genotypes representative of a single clade was found, and these genotypes were common among strains isolated into culture.

TL;DR: Evidence is provided that supports the hypothesis that virus infection can play an important role in determining the success of different Synechococcus genotypes and hence of seasonal succession and multivariate statistical analyses show a significant relationship between cyanophage assemblage structure and that of SyneChococcus.

TL;DR: An in situ hybridization method was applied to the identification of marine cyanobacteria assignable to the genus Prochlorococcus using horseradish-peroxidase-labelled 16S rRNA-targeted oligonucleotide probes in combination with tyramide signal amplification (TSA).

TL;DR: It was demonstrated that PCR products of the correct size could be amplified from seawater samples following 100× concentration and even directly without any prior concentration, and the use of degenerate primers in PCR analyses of cyanophage populations should provide valuable data on the diversity of cyanophile populations in natural assemblages.

TL;DR: It was shown that all six ecotypes had distinct distributions that varied with depth and location, and, with the exception of the deeper waters at the western North Atlantic site, the total Prochlorococcus counts determined by QPCR matched the total counts measured by flow cytometry.