N
Nicholas Muzyczka
Researcher at University of Florida
Publications - 215
Citations - 24920
Nicholas Muzyczka is an academic researcher from University of Florida. The author has contributed to research in topics: Adeno-associated virus & Transduction (genetics). The author has an hindex of 83, co-authored 214 publications receiving 23758 citations. Previous affiliations of Nicholas Muzyczka include State University of New York System & University of North Carolina at Chapel Hill.
Papers
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Journal ArticleDOI
Recombinant adeno-associated virus purification using novel methods improves infectious titer and yield
Sergei Zolotukhin,Barry J. Byrne,E Mason,I Zolotukhin,Mark Potter,Kye Chesnut,C Summerford,Richard Jude Samulski,Nicholas Muzyczka +8 more
TL;DR: Several novel purification strategies that involve the use of non-ionic iodixanol gradients followed by ion exchange or heparin affinity chromatography by either conventional or HPLC columns are reported, which result in more than 50% recovery of rAAV from a crude lysate and routinely produce vector that is more than 99% pure.
Book ChapterDOI
Use of adeno-associated virus as a general transduction vector for mammalian cells.
TL;DR: The ability to establish a latent infection which can later be rescued appears to be a mechanism for ensuring the survival of AAV in the absence of a helper virus.
Journal ArticleDOI
Recombinant AAV viral vectors pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism after delivery to different regions of the central nervous system.
Corinna Burger,Oleg S. Gorbatyuk,Margaret J. Velardo,Carmen S. Peden,Philip J. Williams,Sergei Zolotukhin,Paul J. Reier,Ronald J. Mandel,Nicholas Muzyczka +8 more
TL;DR: The tropism and transduction frequency in the central nervous system of three different rAAV vector serotypes are investigated, suggesting that vectors based on distinct AAV serotypes can be chosen for specific applications in the nervous system.
Journal ArticleDOI
A "humanized" green fluorescent protein cDNA adapted for high-level expression in mammalian cells.
TL;DR: Gfph, a synthetic version of the jellyfish Aequorea victoria green fluorescent protein cDNA that is adapted for high-level expression in mammalian cells, can be readily sorted by fluorescence-activated cell sorting, suggesting that the newly designed gfph gene could be widely used as a reporter in many gene delivery technologies, including human gene therapy.
Patent
AAV transduction vectors
TL;DR: A hybrid gene vector suitable for introducing foreign DNA into a mammalian cell comprising the foreign DNA ligated to an AAV genome was proposed in this article, and a method of constructing the hybrid vector was presented.