Methods for the determination and speciation of mercury in natural waters--a review.

Assessment of Recent Process Analytical Technology (PAT) Trends: A Multiauthor Review

Production of recombinant proteins by yeast cells

This article provides a basic overview of the principles of sequential injection analysis. This is followed by a discussion of the instrumental requirements. A brief overview of some of the diverse analytical applications of SIA is also presented.

Sequential injection analysis

Recombinant therapeutic proteins have gained enormous importance for clinical applications. The first recombinant products have been produced in E. coli more than 20 years ago. Although with the advent of antibody-based therapeutics mammalian expression systems have experienced a major boost, microbial expression systems continue to be widely used in industry. Their intrinsic advantages, such as rapid growth, high yields and ease of manipulation, make them the premier choice for expression of non-glycosylated peptides and proteins. Innovative product classes such as antibody fragments or alternative binding molecules will further expand the use of microbial systems. Even more, novel, engineered production hosts and integrated technology platforms hold enormous potential for future applications. This review summarizes current applications and trends for development, production and analytical characterization of recombinant therapeutic proteins in microbial systems.

Manufacturing of recombinant therapeutic proteins in microbial systems

Multisyringe flow injection analysis coupled to capillary electrophoresis (MSFIA–CE) as a novel analytical tool applied to the pre-concentration, separation and determination of nitrophenols

Flow system device for the on-line determination of total mercury in seawater.

In this paper, the entire publications on modern automation technique multisyringe flow injection analysis (MSFIA) of the last six years are conscientiously reviewed. MSFIA is compared to alternative flow techniques and multisyringe apparatus and its potentials are described in detail. The different injection modalities and manifold configurations as well as analytical applications according to the different detection techniques applied in MSFIA are described and compared. Features and potentials of the related analytical software AutoAnalysis are overviewed and explained on the background of automation examples. The characteristics and features of the analytical methods developed for MSFIA are summarized in a comprehensive overview table.

/pdf/review-on-automation-using-multisyringe-flow-injection-whnojch1yp.pdf

Review on Automation using Multisyringe Flow Injection Analysis

Monitoring of sorbitol in Pichia pastoris cultivation applying sequential injection analysis

Devices and methods for Integrated Bioprocessing have been developed for production of recombinant proteins with the yeast Pichia pastoris. In doing so cross flow filtration techniques for cell separation and product concentration are connected directly to high instrumented cultivation processes. These are equipped with on-line measuring techniques for substrates and products, e.g., glycerol, methanol and pyruvate as well as recombinant proteins, e.g., the chemokines 1-8del MCP-1 and vMIP-II. Complex automation structures allow for process development at virtual plants which can be used as the basis for establishing and implementing fully automated real processes. Experiments for determination of reaction kinetics, optimization of productivity in high-cell density cultures and Integrated Bioprocessing are outlined, along with detailed illustration of the realization of the methods at industrial pilot plant scale.

https://www.onlinelibrary.wiley.com/doi/pdf/10.1002/elsc.200300033

Olaf Elsholz

Papers

Multisyringe flow injection analysis coupled to capillary electrophoresis (MSFIA–CE) as a novel analytical tool applied to the pre-concentration, separation and determination of nitrophenols

Flow system device for the on-line determination of total mercury in seawater.

Review on Automation using Multisyringe Flow Injection Analysis

Monitoring of sorbitol in Pichia pastoris cultivation applying sequential injection analysis

Production of recombinant proteins with Pichia pastoris in integrated processing