Onno Kranenburg

TL;DR: The 911 cell line is demonstrated to have several characteristics in common with the well-known 293 cell line, which make it a useful alternative for the construction, propagation, and titration of E1-deleted recombinant adenoviruses.

TL;DR: This work has identified two cDNAs encoding putative LPA receptors, various LPA-like agonists that act on distinct receptors, and new pathways that link the receptor(s) to such diverse events as Ras signalling, cytoskeletal remodelling and membrane depolarization.

TL;DR: It is shown that p160ROCK activation is necessary and sufficient for the agonist-induced neurite retraction and cell rounding, and Rho/ROCK-dependent tonic inhibition of cell process extension is exerted via activation of the actomysin-based contractility, in conjunction with a suppression of assembly of intermediate filaments and microtubules in many cell types including, but not exclusive to, neuronal cells.

TL;DR: It is shown that overexpression of Tiam1 induces cell spreading and affects neurite outgrowth in N1E-115 neuroblastoma cells and that a balance between these pathways determines neuronal morphology, demonstrating that Rac- and Rho-mediated pathways oppose each other during neurite formation and that

TL;DR: It is concluded that in neuronal cells, LPA activates RhoA to induce growth cone collapse and neurite retraction through a G12/13-initiated pathway that involves protein-tyrosine kinase activity.