Oskar Ortiz

TL;DR: It is revealed for the first time that intein-mediated split–Cas9 can be packaged, delivered and its nuclease activity reconstituted efficiently, in cells via rAAV.

TL;DR: It is reported that CB1 and adenosine A2A receptors form heteromeric complexes in co-transfected HEK-293T cells and rat striatum, where they colocalize in fibrilar structures and counteracted the motor depressant effects produced by the intrastriatal administration of a cannabinoid CB1 receptor agonist.

TL;DR: The results indicate that D(1)R but not D(5)R are critical for hippocampal LTP and for the induction of Zif268 and Arc, proteins required for the transition from E-LTP to L-L TP and for memory consolidation in mammals.

TL;DR: MitoTag mice are engineered, which express a Cre recombinase-dependent green fluorescent protein targeted to the outer mitochondrial membrane, and an isolation approach to profile tagged mitochondria from defined cell types are developed, demonstrating proteomic diversity of cerebellar mitochondria covering bioenergetics, calcium handling and organelle contact sites.

TL;DR: This work presents an advanced approach for the production of mouse disease models by microinjection of transcription activator-like effector nucleases (TALENs) and synthetic oligodeoxynucleotides into one-cell embryos, and enables instant germline modifications, making heterozygous mutants available within 18 wk.