P
P.F. Watson
Researcher at Houston Methodist Hospital
Publications - 2
Citations - 328
P.F. Watson is an academic researcher from Houston Methodist Hospital. The author has contributed to research in topics: Tonicity & Osmotic concentration. The author has an hindex of 2, co-authored 2 publications receiving 307 citations.
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Prevention of osmotic injury to human spermatozoa during addition and removal of glycerol.
Dayong Gao,J. Liu,J. Liu,Chi Liu,L. E. McGann,P.F. Watson,Frederick W. Kleinhans,Frederick W. Kleinhans,Peter Mazur,Peter Mazur,Elizabeth S. Critser,Elizabeth S. Critser,John K. Critser,John K. Critser +13 more
TL;DR: An analytical methodology has been developed for predicting optimal protocols to reduce osmotic injury associated with the addition and removal of hypertonic concentrations of glycerol in human spermatozoa.
Response of spermatozoa to hyposmotic stress reflects cryopreservation success
TL;DR: Ram and boar spermatozoa were progressively disrupted even at mild hyposmotic stress, showing approximately 50% of cells ruptured at 150 mOsm, and human spermutozoa showed a similar response with only a small decrease in intact cells before the precipitous decline at low osmolality.