Chi Liu

TL;DR: An analytical methodology has been developed for predicting optimal protocols to reduce osmotic injury associated with the addition and removal of hypertonic concentrations of glycerol in human spermatozoa.

TL;DR: A cell culture growth substrate comprising submucosal tissue of a warm-blooded vertebrate and a method for culturing eukaryotic cells was described in this article, which supports the proliferation and differentiation of eukarithmic cells when said cells are contacted with sub-mucosa tissue under conditions conducive to cell proliferation.

TL;DR: The use of the microperfusion chamber has the following major advantages: 1) This method allows the extracellular condition(s) to be readily changed by perfusing a single cell or group of cells with a prepared medium (cells can be reperfused with a different medium to study the response of the same cell to different osmotic conditions).

TL;DR: It was concluded that human CD34+CD33- cells osmotically behave as ideal osmometers and this information coupled with cell water and cryoprotectant permeability coefficients as well as their activation energies will be used to design optimum conditions for cryopreservation of human hematopoietic progenitor cells.

TL;DR: Water permeability or hydraulic conductivity and its activation energy, the permeability of the cell plasma membrane to a cryoprotectant(s) (Ps) and its Ea, the osmotically inactive fraction of cell volume (Vb), and the intracellular ice formation temperature were determined for islet cells.