F
Frederick W. Kleinhans
Researcher at Indiana University – Purdue University Indianapolis
Publications - 68
Citations - 3399
Frederick W. Kleinhans is an academic researcher from Indiana University – Purdue University Indianapolis. The author has contributed to research in topics: Cryoprotectant & Cryopreservation. The author has an hindex of 33, co-authored 68 publications receiving 3161 citations. Previous affiliations of Frederick W. Kleinhans include Indiana University & Ohio State University.
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Journal ArticleDOI
Prevention of osmotic injury to human spermatozoa during addition and removal of glycerol.
Dayong Gao,J. Liu,J. Liu,Chi Liu,L. E. McGann,P.F. Watson,Frederick W. Kleinhans,Frederick W. Kleinhans,Peter Mazur,Peter Mazur,Elizabeth S. Critser,Elizabeth S. Critser,John K. Critser,John K. Critser +13 more
TL;DR: An analytical methodology has been developed for predicting optimal protocols to reduce osmotic injury associated with the addition and removal of hypertonic concentrations of glycerol in human spermatozoa.
Journal ArticleDOI
Membrane permeability modeling: Kedem-Katchalsky vs a two-parameter formalism.
TL;DR: This paper demonstrates that the 2P and KK formalism yield essentially the same result (Lp and Ps) when cotransporting channels are absent, and discusses the phenomenological nature of transport parameters in many experiments, especially when both bilayer and channel transport are present.
Journal ArticleDOI
Effect of cryoprotectant solutes on water permeability of human spermatozoa.
J.A. Gilmore,Locksley E. McGann,J. Liu,J. Liu,Dayong Gao,A.T. Peter,Frederick W. Kleinhans,Frederick W. Kleinhans,John K. Critser,John K. Critser,John K. Critser +10 more
TL;DR: Data from this study support the hypothesis that low-molecular-weight, nonionic cryoprotectant solutes affect (decrease) human sperm water permeability, as validated by the use of an electronic particle counter.
Journal ArticleDOI
Hyperosmotic tolerance of human spermatozoa: separate effects of glycerol, sodium chloride, and sucrose on spermolysis.
Dayong Gao,E Ashworth,Paul F. Watson,Frederick W. Kleinhans,Frederick W. Kleinhans,Peter Mazur,J.K. Critser,J.K. Critser +7 more
TL;DR: The results indicate that human spermatozoa exhibited a significant posthypertonic lysis/injury, i.e., loss of membrane integrity, when returned to isosmotic conditions after exposure to hyperosmosis solutions of NaCl or sucrose.
Journal ArticleDOI
Characterization of a Major Permeability Barrier in the Zebrafish Embryo
TL;DR: Zebrafish embryos have two major compartments, the blastoderm and the yolk, which is surrounded by the multinucleated yolk syncytial layer (YSL), and the water and cryoprotectant permeability in these compartments was determined using two methods.