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Parayil Kumaran Ajikumar
Researcher at Massachusetts Institute of Technology
Publications - 57
Citations - 5144
Parayil Kumaran Ajikumar is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Synthetic biology & Metabolic engineering. The author has an hindex of 27, co-authored 57 publications receiving 4553 citations. Previous affiliations of Parayil Kumaran Ajikumar include National University of Singapore & Singapore–MIT alliance.
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Journal Article
Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli
Parayil Kumaran Ajikumar,Wen-Hai Xiao,Keith E. J. Tyo,Yong Wang,Fritz Simeon,Effendi Leonard,Oliver Mucha,Too Heng Phon,Blaine A. Pfeifer,Gregory Stephanopoulos +9 more
TL;DR: In this paper, a multivariate-modular approach to metabolic-pathway engineering that succeeded in increasing titers of taxadiene, the first committed Taxol intermediate, was reported.
Journal ArticleDOI
Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli
Parayil Kumaran Ajikumar,Wen-Hai Xiao,Keith E. J. Tyo,Yong Wang,Fritz Simeon,Effendi Leonard,Oliver Mucha,Too Heng Phon,Blaine A. Pfeifer,Gregory Stephanopoulos,Gregory Stephanopoulos +10 more
TL;DR: A multivariate-modular approach to metabolic-pathway engineering that succeeded in increasing titers of taxadiene—the first committed Taxol intermediate—approximately 1 gram per liter in an engineered Escherichia coli strain helped to unlock the potential of the MEP pathway for the engineered production of terpenoid natural products.
Journal ArticleDOI
Terpenoids: opportunities for biosynthesis of natural product drugs using engineered microorganisms.
Parayil Kumaran Ajikumar,Keith E. J. Tyo,Simon Carlsen,Oliver Mucha,Too Heng Phon,Gregory Stephanopoulos +5 more
TL;DR: This review focuses on the biodiversity of terpenoids, the biosynthetic pathways involved, and engineering efforts to maximize the production through these pathways.
Journal ArticleDOI
Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control
Effendi Leonard,Parayil Kumaran Ajikumar,Kelly M. Thayer,Wen-Hai Xiao,Jeffrey D. Mo,Bruce Tidor,Gregory Stephanopoulos,Kristala L. J. Prather +7 more
TL;DR: The present study highlights the importance of engineering proteins along with pathways as a key strategy in achieving microbial biosynthesis and overproduction of pharmaceutical and chemical products.
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Stabilized gene duplication enables long-term selection-free heterologous pathway expression.
TL;DR: Chemically inducible chromosomal evolution (CIChE), a plasmid-free, high gene copy expression system for engineering Escherichia coli, which improved genetic stability approximately tenfold and growth phase–specific productivity approximately fourfold for a strain producing the high metabolic burden–biopolymer poly-3-hydroxybutyrate.