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Blaine A. Pfeifer
Researcher at State University of New York System
Publications - 132
Citations - 6350
Blaine A. Pfeifer is an academic researcher from State University of New York System. The author has contributed to research in topics: Gene delivery & Polyketide. The author has an hindex of 31, co-authored 131 publications receiving 5575 citations. Previous affiliations of Blaine A. Pfeifer include Tufts University & Massachusetts Institute of Technology.
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Journal Article
Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli
Parayil Kumaran Ajikumar,Wen-Hai Xiao,Keith E. J. Tyo,Yong Wang,Fritz Simeon,Effendi Leonard,Oliver Mucha,Too Heng Phon,Blaine A. Pfeifer,Gregory Stephanopoulos +9 more
TL;DR: In this paper, a multivariate-modular approach to metabolic-pathway engineering that succeeded in increasing titers of taxadiene, the first committed Taxol intermediate, was reported.
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Biosynthesis of Complex Polyketides in a Metabolically Engineered Strain of E. coli
TL;DR: A derivative ofEscherichia coli has been genetically engineered and the resulting cellular catalyst converts exogenous propionate into 6dEB with a specific productivity that compares well with a high-producing mutant of S. erythraea that has been incrementally enhanced over decades for the industrial production of erystromycin.
Journal ArticleDOI
Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli
Parayil Kumaran Ajikumar,Wen-Hai Xiao,Keith E. J. Tyo,Yong Wang,Fritz Simeon,Effendi Leonard,Oliver Mucha,Too Heng Phon,Blaine A. Pfeifer,Gregory Stephanopoulos,Gregory Stephanopoulos +10 more
TL;DR: A multivariate-modular approach to metabolic-pathway engineering that succeeded in increasing titers of taxadiene—the first committed Taxol intermediate—approximately 1 gram per liter in an engineered Escherichia coli strain helped to unlock the potential of the MEP pathway for the engineered production of terpenoid natural products.
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Overcoming Nonviral Gene Delivery Barriers: Perspective and Future
TL;DR: This work presents the existing delivery barriers and summarize current vector-specific strategies to overcome said barriers, and focuses on the gene delivery barriers associated with the two most common classes of nonviral vectors, cationic-based lipids and polymers.
Journal ArticleDOI
Porphyrin–phospholipid liposomes permeabilized by near-infrared light
Kevin A. Carter,Shuai Shao,Matthew I. Hoopes,Dandan Luo,Bilal Ahsan,Vladimir M. Grigoryants,Wentao Song,Haoyuan Huang,Guojian Zhang,Ravindra K. Pandey,Jumin Geng,Blaine A. Pfeifer,Charles P. Scholes,Joaquin Ortega,Mikko Karttunen,Jonathan F. Lovell +15 more
TL;DR: Liposomes doped with porphyrin–phospholipid that are permeabilized directly by near-infrared light are described and spatial and temporal control of release of entrapped fluorophores following intratumoral injection are demonstrated.