P
Patrick Martin
Researcher at Martin Luther University of Halle-Wittenberg
Publications - 7
Citations - 113
Patrick Martin is an academic researcher from Martin Luther University of Halle-Wittenberg. The author has contributed to research in topics: Gene & Mutant. The author has an hindex of 3, co-authored 7 publications receiving 36 citations.
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Journal ArticleDOI
High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns
Ramona Grützner,Patrick Martin,Claudia Horn,Samuel Mortensen,Erin J. Cram,Carolyn W. T. Lee-Parsons,Johannes Stuttmann,Sylvestre Marillonnet +7 more
TL;DR: To optimize the efficiency of the Cas9 nuclease in generating mutations in target genes in Arabidopsis thaliana, several features of its nucleotide and/or amino acid sequence are investigated, including the codon usage, the number of nuclear localization signals (NLSs), and the presence or absence of introns.
Journal ArticleDOI
Highly efficient multiplex editing: one-shot generation of 8× Nicotiana benthamiana and 12× Arabidopsis mutants
Johannes Stuttmann,Karen Barthel,Patrick Martin,Jana Ordon,Jessica L. Erickson,Rosalie Herr,Filiz Ferik,Carola Kretschmer,Thomas Berner,Jens Keilwagen,Sylvestre Marillonnet,Ulla Bonas +11 more
TL;DR: In this article, a toolkit based on a highly intron-optimized zCas9i gene was developed, which allows assembly of nuclease constructs expressing up to 32 single guide RNAs (sgRNAs).
Posted ContentDOI
One-shot generation of duodecuple (12x) mutant Arabidopsis: Highly efficient routine editing in model species
Karen Barthel,Patrick Martin,Jana Ordon,Jessica L. Erickson,Johannes Gantner,Rosalie Herr,Carola Kretschmer,Thomas Berner,Jens Keilwagen,Sylvestre Marillonnet,Johannes Stuttmann +10 more
TL;DR: A toolkit based on an intron-optimized SpCas9-coding gene (zCas9i), which conveys dramatically enhanced editing efficiencies and reveals new perspectives for multiplexing to target gene families and to generate higher order mutants is presented.
Journal ArticleDOI
Disentangling cause and consequence: genetic dissection of the DANGEROUS MIX2 risk locus, and activation of the DM2h NLR in autoimmunity.
Jana Ordon,Patrick Martin,Jessica L. Erickson,Filiz Ferik,Gerd Ulrich Balcke,Ulla Bonas,Johannes Stuttmann +6 more
TL;DR: It is proposed that autoimmunity may, at least in some cases, be caused by spurious, stochastic interactions of foreign alleles with co-incidentally matching risk NLRs.
Posted ContentDOI
Highly efficient multiplex editing: One-shot generation of 8x Nicotiana benthamiana and 12x Arabidopsis mutants
Johannes Stuttmann,Karen Barthel,Patrick Martin,Jana Ordon,Jessica L. Erickson,Rosalie Herr,Filiz Ferik,Carola Kretschmer,Thomas Berner,Jens Keilwagen,Sylvestre Marillonnet,Ulla Bonas +11 more
TL;DR: In this paper, a toolkit based on a highly intron-optimized zCas9i gene was developed, which allows assembly of nuclease constructs expressing up to 32 sgRNAs.