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Showing papers by "Paul F. Greenfield published in 1989"


Journal ArticleDOI
TL;DR: The name Nocardia pinensis has been chosen for the new nocardioform actinomycete because of the pine tree like appearance of the organism on microscopy.
Abstract: A new nocardioform actinomycete was isolated by filament micromanipulation during the course of a study into foaming in activated sludge plants in Australia. It constitutes the second most prevalent foaming organism in Australia after Nocardia amarae. These two foaming organisms can be differentiated morphologically, biochemically and chemotaxonomically. The microscopic appearance of the filaments of the new taxon resembles a pine tree. The filaments are Gram-positive, non-acid-fast, non-motile, non-sheathed and about 0·5-1·0 μm in diameter. On a complex medium, the colonies are orange, opaque, macroscopically dry and friable, microscopically moist and shiny, with a pasty texture and an entire edge. The strains are positive for catalase, oxidase and urease and are oxidative in their metabolism of glucose. No strain could degrade hypoxanthine, xanthine, tyrosine, casein, gelatin or aesculin and none could grow with lysozyme. The strains contain peptidoglycan type Aly, cell wall type IV, whole cell sugar pattern type A, phospholipid type PII, menaquinones ω-cyclo-MK-8 (H4), fatty acids comprising straight chain saturated and unsaturated acids and tuberculostearic acid, and mycolic acids with 58-64 carbons containing substantial amounts of unsaturated chains in the 2-position. The name Nocardia pinensis has been chosen for the new taxon because of the pine tree like appearance of the organism on microscopy. The type strain, UQM3063, is deposited at the University of Queensland, Department of Microbiology Culture Collection.

59 citations


Journal ArticleDOI
TL;DR: In this article, the main effects of nutrients on corals appear to be indirect and the available evidence implies that denitrification and phosphorus removal are necessary treatment requirements if acceptable levels (after dilution) of these components are to be achieved.

34 citations


Journal ArticleDOI
TL;DR: The fraction of plasmid containing cells was found to decrease more rapidly after a step change to low dissolved oxygen levels, and an increased rate ofplasmid loss appeared to be the major cause of the decreased stability.
Abstract: The effect of oxygen limitation on the stability of a recombinant yeast plasmid was measured during continuous culture in a non-selective medium. The fraction of plasmid containing cells was found to decrease more rapidly after a step change to low dissolved oxygen (DOT) levels. An increased rate of plasmid loss appeared to be the major cause of the decreased stability.

33 citations


Journal ArticleDOI
TL;DR: Membrane filters overlaid on slabs of a model solid substrate enabled recovery of biomass of Rhizopus oligosporus and provides a useful tool for studying solid-state fermentation.
Abstract: Membrane filters overlaid on slabs of a model solid substrate enabled recovery of biomass ofRhizopus oligosporus. Although the presence of the membrane filter affects the growth ofRhizopus oligosporus it provides a useful tool for studying solid-state fermentation.

25 citations


Journal ArticleDOI
TL;DR: In this article, the improved effluent control of industrial anaerobic digestion treatment systems is described, and a modification to the existing algorithm, based on a new control technique known as Generic Model Control, that simplifies and improves the robustness of the control algorithm is presented.
Abstract: This paper describes the improved effluent control of industrial anaerobic digestion treatment systems. Previous research has shown the possibility of applying a model-based adaptive control technique to these processes. This paper reviews this technique and shows that improved controllability can be obtained by a modification to the existing algorithm, based on a new control technique known as Generic Model Control, that simplifies and improves the robustness of the control algorithm.

8 citations