Showing papers by "Paul Pui-Hay But published in 2012"
TL;DR: The semiquantitative reverse transcription polymerase chain reaction assay suggested that trans-ethyl p-methoxycinnamate affects multiple molecular targets related to angiogenesis and could block bFGF-induced vessel formation on Matrigel plug assay.
Abstract: Kaempferia galanga L. (Zingiberaceae) is an aromatic herb and a popular spice used as a condiment in Asian cuisine. The ethanol extract of the dried plant and its successive four subfractions were investigated on zebrafish model by quantitative endogenous alkaline phosphatase assay. Both n-hexane and ethyl acetate fractions had antiangiogenic activity, and two major active components (trans-ethyl p-methoxycinnamate and kaempferol) showed potent antiangiogenic effects on wild-type zebrafish. Because of its much stronger effect and no antiangiogenic activity reported, trans-ethyl p-methoxycinnamate was further investigated for its action mechanism. It dose dependently inhibited vessel formation on both wild- and Tg(fli1a:EGFP)y1-type zebrafish embryos. The semiquantitative reverse transcription polymerase chain reaction assay suggested that trans-ethyl p-methoxycinnamate affects multiple molecular targets related to angiogenesis. In vitro, it specifically inhibited the migration and tube formation of human umbilical vein endothelial cells. In vivo, it could block bFGF-induced vessel formation on Matrigel plug assay.
37 citations
TL;DR: This is the first study applying an integrated molecular, chemical and biological approach to differentiate traditional Chinese medicine from Aristolochia adulterant.
28 citations
TL;DR: Two molecular tools are applied to authenticate abalone products and multiplex PCR with a Haliotis-specific primer was used to facilitate rapid and reliable exclusion of substitutes from abalone Products.
22 citations
TL;DR: Investigation of dried fruits of Alpinia caerulea (R.Br.) Bentham, a perennial herb growing in tropical and subtropical Australia, revealed that zerumin A specifically inhibited the proliferation and migration steps, to prevent angiogenesis progress.
22 citations
TL;DR: This is the first study using DNA barcoding techniques to identify the Chinese ‘cooling’ beverage materials and the gathered DNA barcodes are useful asset for standardization and quality control purposes.
16 citations
TL;DR: This review provides an up-to-date coverage of scientific advances in species identification and authentication among the three genuine species and substitutes derived from the genus Asparagus, characterization of the Stemona alkaloids, and development of quality assessment methods.
Abstract: The Chinese herb Radix Stemonae, or Baibu in Chinese, has been used for treating coughing for over 15 centuries. According to the Pharmacopoeia of the People's Republic of China, it comes from the tuberous roots of three species of Stemona . The properties of multiple origins of source plants and existence of multiple antitussive components made the authentication and quality assessment extremely difficult. This review provides an up-to-date coverage of scientific advances in species identification and authentication among the three genuine species and substitutes derived from the genus Asparagus , characterization of the Stemona alkaloids including chemical diversities among the three genuine species and among the major commodity derived from Stemona tuberosa from different locations, identification of the antitussive components and mechanisms of action, clarification of adverse reactions, and, based on all these information, development of quality assessment methods.
11 citations
TL;DR: The success of FINS identification depends on the DNA loci as they show different abilities to differentiate genuine species from substitutes, to discriminate closely related species and to generate quality sequences.
2 citations