Showing papers by "Paweł Pomastowski published in 2017"
TL;DR: The methods of synthesizing zinc oxide nanocomposites as well as their characteristics, antimicrobial activity and cytotoxicity against normal and tumor cells are reviewed.
398 citations
TL;DR: This review draws attention to the necessity of developing modern analytical tools for identification and quantification of individual M. sativa phytochemicals.
70 citations
TL;DR: An approach using capillary electrophoresis of microbial aggregates with UV and matrix-assisted laser desorption ionization time-of-flight MS detection is presented, highlighting several challenges with the analysis of microbial complexes using electromigration technology.
Abstract: Rapid detection and identification of microorganisms is a challenging and important aspect in a wide range of fields, from medical to industrial, affecting human lives. Unfortunately, classical methods of microorganism identification are based on time-consuming and labor-intensive approaches. Screening techniques require the rapid and cheap grouping of bacterial isolates; however, modern bioanalytics demand comprehensive bacterial studies at a molecular level. Modern approaches for the rapid identification of bacteria use molecular techniques, such as 16S ribosomal RNA gene sequencing based on polymerase chain reaction or electromigration, especially capillary zone electrophoresis and capillary isoelectric focusing. However, there are still several challenges with the analysis of microbial complexes using electromigration technology, such as uncontrolled aggregation and/or adhesion to the capillary surface. Thus, an approach using capillary electrophoresis of microbial aggregates with UV and matrix-assisted laser desorption ionization time-of-flight MS detection is presented.
51 citations
TL;DR: The diatom frustules possess a three-dimensional structure with silica walls perforated by spatially periodic porous network and can be considered as naturally organic functionalized 3D silica composite.
45 citations
TL;DR: The methodologies employed for sampling, extraction and enrichment of salivary VOCs are presented and the literature dedicated to profiling and searching of biomarkers emanated from saliva is reviewed.
Abstract: Saliva is an aqueous fluid found in the oral cavity playing fundamental role in the preservation and maintenance of oral health. Apart from higher molecular-weight constituents, it contains volatile organic compounds (VOCs) that may origin from such sources such as blood or be a result of bacterial metabolism. They can also reach the oral fluid through dissolving of volatiles from breath and consumed foods. Some of them may serve as biomarkers of various diseases, such as dimethyl disulfide (halitosis), pyridine (periodontal disease) and trimethylamine (trimethylaminuria) or occupational exposure. The review shows the basic functions of saliva and its composition. Furthermore, this paper presents the methodologies employed for sampling, extraction and enrichment of salivary VOCs and reviews the literature dedicated to profiling and searching of biomarkers emanated from saliva. Complete tables of reported VOCs and potential biomarkers from this specimen are also included.
34 citations
TL;DR: The antibacterial activity of biologically synthesized nanoparticles functionalized with ampicillin against a range of gram (+) and gram (−) bacteria strains such as Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphlyococcus epidermidis, and Escherichia coli is assessed.
Abstract: The resistance of pathogenic bacteria to antibiotics has become a serious problem. The emphasis is placed on the development of new, effective antimicrobial strategies. One of them is the use of AgNPs in association with antibiotic drugs. The aim of this study was to obtain silver nanoparticles functionalized with ampicillin and to investigate the mechanism of binding antibiotics to nanoparticle using high-performance liquid chromatography approach. To confirm the occurrence of silver nanoparticles functionalization, FTIR, MALDI-TOF MS, and DLS analysis and zeta potential measurements were performed. Moreover we assessed the antibacterial activity of biologically synthesized nanoparticles functionalized with ampicillin against a range of gram (+) and gram (-) bacteria strains such as Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus epidermidis, and Escherichia coli.
28 citations
TL;DR: The mechanism of sorption of silver cations by Lactococcus lactis and Lactobacillus casei bacteria was investigated and an analysis of volatile organic compounds extracted from bacterial cells was performed.
Abstract: The current work deals with the phenomenon of silver cations uptake by two kinds of bacteria isolated from dairy products. The mechanism of sorption of silver cations by Lactococcus lactis and Lactobacillus casei bacteria was investigated. Inductively coupled plasma–mass spectrometry (ICP-MS) was used for determination of silver concentration sorbed by bacteria. Analysis of charge distribution was conducted by diffraction light scattering method. Changes in the ultrastructure of Lactococcus lactis and Lactobacillus casei cells after treatment with silver cations were investigated using transmission electron microscopy observation. Molecular spectroscopy methods, namely Fourier transform-infrared spectroscopy (FT-IR) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) were employed for description of the sorption mechanism. Moreover, an analysis of volatile organic compounds (VOCs) extracted from bacterial cells was performed.
23 citations
TL;DR: The elaborated novel procedure of HEMA-MBA hydrophilic monolith preparation allowed for the introduction of higher monomers content compared to earlier literature data.
21 citations
TL;DR: Silver nanoparticles synthesized by Actinomycetes HGG 16n strain were as effective antimicrobial agent as the tested commercial antibiotics and were more sensitive than disc and well diffusion and confirmed the effectiveness of BioAgNPs against all tested bacterial cells.
Abstract: Background: Biologically synthetized silver nanoparticles are promising antimicrobial agent. Flow cytometry, well diffusion methods, colony-forming units (CFU) and spectroscopic approach are commonly used in antimicrobial study. The aim of this study was investigation of effectiveness of Bio- AgNPs synthesized by Actinomycetes HGG16n using fluorescence flow cytometry method as an alternative to the standard ones (well and disc diffusion method). Methods: Flow cytometry technique was applied to monitor the antibacterial effect of biocolloidal silver and its combination with various commercial antibiotics against selected pathogens. The observed effectiveness was confirmed by fluorescence micrographs. Result: Silver nanoparticles synthesized by Actinomycetes HGG 16n strain were as effective antimicrobial agent as the tested commercial antibiotics. Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Salmonella infantis and Bacillus subtilis strains are not able to create the resistant mechanisms under treatment of biocolloidal silver. Furthermore, the flow cytometry technique was more sensitive than disc and well diffusion and confirmed the effectiveness of BioAgNPs against all tested bacterial cells. Precipitation and limited diffusion of biocolloidal silver was observed by using well diffusion method. Conclusion: Specificity and selection of antimicrobial approach are related with different nature of lowmolecular compounds (e.g. antibiotic) compared with biocolloids. An alternative method, flow cytometry was designed for antimicrobial study of biocolloidal silver nanoparticles and compared to the classical microbial techniques. Moreover, this work highlights the development of novel and inexpensive antimicrobial agent for most oral and skin infections caused by Gram (+) and Gram (-) bacteria.
14 citations
TL;DR: Oral bacteria were the most viable within 2 h after collection of saliva and the most prevalent strain was Sphingomonas paucimobilis, but HS‐SPME‐GC/MS method is insufficient for volatile profiling of “fresh” saliva and should be directed rather to investigation of bacterial metabolites.
Abstract: The aim of the study was determination of bacterial viability in saliva samples and finding a correlation between microbiological and volatile profiles of saliva depending on incubation time. Bacteria colonizing healthy oral cavities were also identified. Twelve healthy adults donated unstimulated saliva samples. Flow cytometry, optical density measurements and colony-forming unit (CFU) counting method were employed for analyses of native and inoculated saliva after 0, 1, 2, 24 and 48 h of incubation. Volatile profiles were acquired using headspace-solid phase microextraction-gas chromatography/mass spectrometry (HS-SPME-GC/MS). Oral bacteria were the most viable within 2 h after collection of saliva. Extension of incubation time to 48 h caused considerable decrease in live bacteria counts and sharp increase in dead bacteria counts. The most prevalent strain was Sphingomonas paucimobilis (26.67%). The number of volatiles raised from 5 to 27 with incubation time and most of them were putrefaction products, such as methanethiol, indole and pyrrole. HS-SPME-GC/MS method is insufficient for volatile profiling of “fresh” saliva and should be directed rather to investigation of bacterial metabolites.
This article is protected by copyright. All rights reserved
7 citations