Showing papers by "Per A. Peterson published in 1993"
TL;DR: Differences in the subcellular distribution and rate of post-translational modification of CD8 maintained in the ER by sequences derived from a variety of ER resident proteins suggested that the efficiency of retrieval was dependent on the sequence context of the double lysine motif and that retrieval may be initiated from multiple positions along the exocytotic pathway.
Abstract: A COOH-terminal double lysine motif maintains type I transmembrane proteins in the ER. Proteins tagged with this motif, eg., CD8/E19 and CD4/E19, rapidly receive post-translational modifications characteristic of the intermediate compartment and partially colocalized to this organelle. These proteins also received modifications characteristic of the Golgi but much more slowly. Lectin staining localized these Golgi modified proteins to ER indicating that this motif is a retrieval signal. Differences in the subcellular distribution and rate of post-translational modification of CD8 maintained in the ER by sequences derived from a variety of ER resident proteins suggested that the efficiency of retrieval was dependent on the sequence context of the double lysine motif and that retrieval may be initiated from multiple positions along the exocytotic pathway.
405 citations
69 citations
TL;DR: Compared the affinities of various octapeptides for purified, soluble H-2Kb molecules revealed that at least 2 anchor residues are necessary for high-affinity binding, and that high-Affinity binding occurs only when anchor side chains are optimally packed within the groove.
66 citations
Patent•
18 Feb 1993TL;DR: In this paper, a rational, elegant means of producing, loading and using Class I molecules to specifically activate CD8 cells in vitro, and their therapeutic applications in the treatment of a variety of conditions, including cancer, tumors or neoplasias, as well as viral, retroviral, autoimmune, and autoimmune type diseases.
Abstract: The present invention relates to a rational, elegant means of producing, loading and using Class I molecules to specifically activate CD8 cells in vitro, and their therapeutic applications in the treatment of a variety of conditions, including cancer, tumors or neoplasias, as well as viral, retroviral, autoimmune, and autoimmune-type diseases. The present invention also relates to vectors, cell lines, recombinant DNA molecules encoding human β2 microglobulin or Class I MHC molecules in soluble and insoluble form, and methods of producing same.
46 citations
01 Jan 1993
TL;DR: Proteins destined for the exocytic pathway are directed into the endoplasmic reticulum by an N-terminal signal peptide, and when transplanted onto reporter proteins, these motifs maintain the chimeric proteins in the ER.
Abstract: Proteins destined for the exocytic pathway are directed into the endoplasmic reticulum (ER) by an N-terminal signal peptide. Current views support the idea that transport occurs by default such that proteins inserted into the ER are carried passively to the cell surface unless retained by structural motifs (Pfeffer and Rothman 1987). In the case of ER resident proteins, such motifs have been identified for both lumenal and type I transmembrane proteins (Munro and Pelham 1987, Jackson et al 1990). When transplanted onto reporter proteins, these motifs maintain the chimeric proteins in the ER.
1 citations