Showing papers in "Journal of Biological Chemistry in 1993"

TL;DR: In vitro kinase assays indicated that interference with Ca2+ or its binding site is not responsible for the differential inhibition of PKC isozymes by Gö 6976, and Kinetic analysis revealed that PKC inhibition by Gö 7076 was competitive with respect to ATP, non-competitive withrespect to the protein substrate, and mixed type with respectto phosphatidylserine.

TL;DR: It is shown that all sHsps investigated act as molecular chaperones in these folding reactions and at stoichiometric amounts they maximally prevent the aggregation of citrate synthase and alpha-glucosidase under heat shock conditions and stabilize the proteins.

TL;DR: Results demonstrate that the two PGH synthases are pharmacologically distinct and indicate that it may be possible to develop isozyme-specific cyclooxygenase inhibitors useful both for anti-inflammatory therapy and for delineating between the biological roles of the P GH synthase isozymes.

TL;DR: The experimental findings with the mutant receptor cannot be adequately rationalized within the theoretical framework of the Ternary Complex Model, and an extended version of this model that includes an explicit isomerization of the receptor to an active state closely models all the findings for both the mutant and the wild-type receptors.

TL;DR: CD36 may play a role in scavenging LDL modified by oxidation and may mediate effects of OxLDL on monocytes and platelets in atherosclerotic lesions.

TL;DR: A 25-kDa protein was found to be associated with purified human neutrophil gelatinase, which showed a high degree of similarity with the deduced sequences of rat alpha 2-microglobulin-related protein and the mouse protein 24p3, and is a new member of the lipocalin family.

TL;DR: Northern blotting and in situ hybridization analyses revealed that the expressions of individual mRNAs for the NMDAR2 subunits overlap in some brain regions but are also specialized in many other regions.

TL;DR: The data suggest that FAT and CD36 belong to a family of proteins that bind/transport long-chain fatty acids or function as regulators of these processes.

TL;DR: A 68-amino acid portion of the signal-transducing domain significantly conserved in the Fas antigen as well as in the type I tumor necrosis factor receptor was considered to be the novel protein domain required for apoptotic signal transduction.

TL;DR: The results suggest that PKC zeta may be a target for PIP3 and thus may be involved in the signaling mechanism(s) for growth factors and oncogenes that increase phosphatidylinositol 3-kinase activity.

TL;DR: Exendin-4 is an agonist and exendin-(9-39)-amide is a specific GLP-1 receptor antagonist, and both peptides stimulated the proinsulin gene expression at the level of transcription and reduced the effects of cAMP.

TL;DR: It is demonstrated that induction of both Hif-1 and erythropoietin RNA is inhibited by the protein kinase inhibitor 2-aminopurine, consistent with the proposed function of HIF-1 as a physiologic regulator of gene expression that responds to changes in cellular oxygen tension.

TL;DR: The cloning, expression, and gene localization of a fourth human melanocortin receptor, the melanocortex-4 receptor, is reported, which is expressed primarily in the brain, but its expression is notably absent in the adrenal cortex, melanocytes, and placenta.

TL;DR: Characterization of 5'-flanking genomic regions indicates that the endothelial NO synthase promoter is "TATA-less" and exhibits proximal promoter elements consistent with a constitutively expressed gene that is found in endothelial cells, namely Sp1 and GATA motifs.

TL;DR: The use of a previously described 17-mer phosphorothioate for structure-function analysis of 2'-sugar modifications and the results demonstrate the importance of target affinity in the action of antisense oligonucleotides and of RNase H as a mechanism by which these compounds exert their effects.

TL;DR: In this article, a rat fetus expression library was used to clone a 363-amino acid protein with pharmacological specificity, tissue distribution, and developmental pattern of the AT2 receptor with 34% identical in sequence to the AT1 receptor, sharing a seven-transmembrane domain topology.

TL;DR: The abundance of structurally altered aspartyl residues may profoundly affect the conformation of the beta A protein within plaque cores and thus significantly impact normal catabolic processes designed to limit its deposition, and contribute to the production and stability of beta-amyloid deposits in Alzheimer brain tissue.

TL;DR: The results suggest that PI3-kinase is involved in the signal transduction pathway responsible for histamine secretion following stimulation of Fc epsilon RI and that wortmannin blocks these responses through direct interaction with the catalytic subunit of this enzyme.

TL;DR: The molecular cloning of human PHS type II from an endothelial cell cDNA library is reported and it is concluded that expression of PHS II may have important pathophysiological effects in the vasculature.

TL;DR: Blot and in situ hybridization analyses indicated that mGluR6 mRNA is restrictedly expressed in the inner nuclear layer of the retina where ON-bipolar cells are distributed, and the physiological role of this receptor subtype in the visual system is discussed.

TL;DR: The molecular cloning and pharmacologic characterization of a third member of this receptor family is reported, which characterizes it as an MSH receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH.

TL;DR: The expressed AT2 receptor mediated angiotensin II-induced inhibition of protein tyrosine phosphatase, an action that was dependent on a pertussis toxin-sensitive G-protein-coupled mechanism in COS-7 cells, and suggested novel functional roles of the renin-angiotens in system in cross-talk with phosphotyrosine signaling by modulating protein phosphotYrosine levels.

TL;DR: The findings suggest that the abnormal phosphorylation of tau in AD occurs in the cytosol.

TL;DR: Results indicate that P-glycoprotein transports the immunosuppressive agents cyclosporin A and FK506.

TL;DR: The hypothesis that ceramide is a specific lipid second messenger modulating heterotrimeric PP2A activity is supported.

TL;DR: The expression of HC gp-39 may be related to a response of human articular cartilage cells to an altered tissue environment, and significant homologies were observed with three mammalian secretory proteins of as yet unknown function, suggesting that a related protein family exists in mammals.

TL;DR: The working hypothesis is O2- inactivates these enzymes by oxidizing their clusters to an unstable oxidation state, and cluster degradation follows, and spinach dihydroxy-acid dehydratase, a member of the hydro-lyase class that has a catalytically active [2Fe-2S] cluster, is not inactivated and does not lose iron in the presence of O2.