P
Petra Siegert
Researcher at FH Aachen
Publications - 96
Citations - 1915
Petra Siegert is an academic researcher from FH Aachen. The author has contributed to research in topics: Subtilisin & Protease. The author has an hindex of 23, co-authored 88 publications receiving 1794 citations. Previous affiliations of Petra Siegert include University of Düsseldorf & Bosch.
Papers
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Journal ArticleDOI
Development of a donor-acceptor concept for enzymatic cross-coupling reactions of aldehydes: the first asymmetric cross-benzoin condensation.
Pascal Dünkelmann,Doris Kolter-Jung,Adam Nitsche,Ayhan S. Demir,Petra Siegert,Bettina Lingen,Martin Baumann,Martina Pohl,Michael Müller +8 more
TL;DR: Highly enantioenriched mixed benzoins are obtained selectively through a biocatalytical cross-coupling reaction of aromatic aldehydes using ThDP-dependent enzymes.
Journal ArticleDOI
Benzoylformate Decarboxylase from Pseudomonas putida as Stable Catalyst for the Synthesis of Chiral 2‐Hydroxy Ketones
Hans Iding,Thomas Dünnwald,Lasse Greiner,Andreas Liese,Michael Müller,Petra Siegert,Joachim Grötzinger,Ayhan S. Demir,Martina Pohl +8 more
TL;DR: A new enzyme tool comprising a vast applicability for stereoselective synthesis and a simple way to increase the enantiomeric excess of (S)-2-hydroxy-1-phenyl-propanone from 90% to 95% by skillful choice of the reaction parameters is presented.
Journal ArticleDOI
Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida.
Petra Siegert,Michael J. McLeish,Martin Baumann,Hans Iding,Malea M. Kneen,George L. Kenyon,Martina Pohl +6 more
TL;DR: Comparison of the X-ray structures of both enzymes identified two residues in each that were likely to be involved in determining substrate specificity that proved to be a real chimera between PDC and BFD and provided the most interesting result with an almost complete reversal of the stereochemistry of its 2-hydroxypropiophenone product.
Journal ArticleDOI
Hydrolysis of polyethyleneterephthalate by p-nitrobenzylesterase from Bacillus subtilis.
Doris Ribitsch,Sonja Heumann,Eva Trotscha,Enrique Herrero Acero,Katrin Greimel,Regina Leber,Ruth Birner-Gruenberger,Sigrid Deller,Inge Eiteljoerg,Peter Remler,Thomas Weber,Petra Siegert,Karl-Heinz Maurer,Ilaria Donelli,Giuliano Freddi,Helmut Schwab,Georg M. Guebitz +16 more
TL;DR: PET‐hydrolase active strains produced clearing zones and led to the release of the 3PET hydrolysis products terephthalic acid (TA), benzoic Acid (BA), 2‐Hydroxyethyl benzoate (HEB), and mono‐(2‐hydroxyethyl) terecularate (MHET) in 3PET supplemented liquid cultures.
Patent
Detergents and cleaners comprising proteases from xanthomonas
TL;DR: In this article, the detergency of detergents and cleaners was improved by adding a protease from a bacterium of the genus Xanthomonas, which was used to improve the detergent detergence.