Philippe Clertant

TL;DR: Examining whether live vaccinia virus recombinants expressing TSA in cells of the vaccinated host might better elicit tumour immunity concluded that tumour-bearing animals could be induced to reject their tumours by inoculation of recombinant.

TL;DR: The observations suggest that the papillomavirus E1 proteins might have a function in common with the polyoma virus large-T proteins (which are required for the initiation of viral DNA replication), which are general characteristics of eukaryotic proteins involved in the control of DNA replication.

TL;DR: The interactions between E1 and DNA polymerase alpha-primase do not exhibit cell-type specificity, as had already been suggested by data from in vivo and in vitro replication assays, but they imply that other cellular proteins may affect the level of E1-dependent replication.

TL;DR: It is reported that adding E2 to cell-free assays produced only slight effects both on the yield of E1-dependent DNA synthesis and on the quality of newly made DNA molecules when a template carrying a wild-type BPV1 replication origin (ori) was used, suggesting that during the initiation of bovine papillomavirus type 1 DNA replication, at least in vitro, E2 acts as a specificity factor restricting the action of E 1 to a defined ori sequence.

TL;DR: Bovine papillomavirus E1 protein was found to be as efficient as the simian virus 40 large T antigen in initiating DNA synthesis in a cell-free system derived from COS1 cells, therefore, E1 functions in vitro as a lytic virus initiator.