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Phyllis Biddle
Researcher at DuPont
Publications - 4
Citations - 1382
Phyllis Biddle is an academic researcher from DuPont. The author has contributed to research in topics: Rhizoctonia solani & Chitinase. The author has an hindex of 4, co-authored 4 publications receiving 1362 citations.
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Journal ArticleDOI
Transgenic Plants with Enhanced Resistance to the Fungal Pathogen Rhizoctonia solani
Karen Brogue,Ilan Chet,Mark Holliday,Robert F. Cressman,Phyllis Biddle,Susan Knowlton,C. Jeffry Mauvais,Richard Broglie +7 more
TL;DR: Transgenic tobacco seedlings constitutively expressing a bean chitinase gene under control of the cauliflower mosaic virus 35S promoter showed an increased ability to survive in soil infested with the fungal pathogen Rhizoctonia solani and delayed development of disease symptoms.
Journal ArticleDOI
Functional analysis of DNA sequences responsible for ethylene regulation of a bean chitinase gene in transgenic tobacco.
TL;DR: Ethylene-dependent expression of a chimeric gene consisting of 1.6 kilobases of 5'-flanking DNA derived from the CH5B gene fused to the coding sequence of beta-glucuronidase indicates that this region of the CH 5B gene is sufficient for ethylene-regulated expression.
Journal ArticleDOI
Activation of a Bean Chitinase Promoter in Transgenic Tobacco Plants by Phytopathogenic Fungi.
TL;DR: Analysis of transgenic tobacco plants containing a chimeric gene composed of a 1.7-kilobase fragment carrying the chitinase 5B gene promoter fused to the coding region of the gus A gene indicated that the ch itinase promoter is activated during attack by the fungal pathogens Botrytis cinerea, Rhizoctonia solani, and Sclerotium rolfsii.
Journal ArticleDOI
Rapid genome-wide single nucleotide polymorphism discovery in soybean and rice via deep resequencing of reduced representation libraries with the illumina genome analyzer.
Stéphane Deschamps,Mauricio La Rota,Jeffrey P. Ratashak,Phyllis Biddle,Dean L. Thureen,Andrew Farmer,Stanley Luck,Mary Beatty,Nobuhiro Nagasawa,Leah Michael,Victor Llaca,Hajime Sakai,Gregory D. May,Jonathan Lightner,Matthew A. Campbell +14 more
TL;DR: The creation of reduced representation libraries (RRLs) using an initial digestion of nuclear genomic DNA with a methylation‐sensitive restriction endonuclease followed by a secondary digestion with the 4bp‐restriction end onuclease DpnII allows for the enrichment of hypomethylated genomic DNA.