R
Richard Broglie
Researcher at DuPont
Publications - 12
Citations - 1687
Richard Broglie is an academic researcher from DuPont. The author has contributed to research in topics: Rhizoctonia solani & Chitinase. The author has an hindex of 10, co-authored 12 publications receiving 1607 citations.
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Journal ArticleDOI
Transgenic Plants with Enhanced Resistance to the Fungal Pathogen Rhizoctonia solani
Karen Brogue,Ilan Chet,Mark Holliday,Robert F. Cressman,Phyllis Biddle,Susan Knowlton,C. Jeffry Mauvais,Richard Broglie +7 more
TL;DR: Transgenic tobacco seedlings constitutively expressing a bean chitinase gene under control of the cauliflower mosaic virus 35S promoter showed an increased ability to survive in soil infested with the fungal pathogen Rhizoctonia solani and delayed development of disease symptoms.
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Functional analysis of DNA sequences responsible for ethylene regulation of a bean chitinase gene in transgenic tobacco.
TL;DR: Ethylene-dependent expression of a chimeric gene consisting of 1.6 kilobases of 5'-flanking DNA derived from the CH5B gene fused to the coding sequence of beta-glucuronidase indicates that this region of the CH 5B gene is sufficient for ethylene-regulated expression.
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Molecular characterization of a novel β-1,3-exoglucanase related to mycoparasitism of Trichoderma harzianum
TL;DR: A 110kDa novel extracellular beta-1,3-exoglucanase from T. harzianum is purified and contains a unique C-terminal embodying cysteine motifs, enabling Trichoderma to utilize both their cell walls and cellular contents for nutrition.
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Activation of a Bean Chitinase Promoter in Transgenic Tobacco Plants by Phytopathogenic Fungi.
TL;DR: Analysis of transgenic tobacco plants containing a chimeric gene composed of a 1.7-kilobase fragment carrying the chitinase 5B gene promoter fused to the coding region of the gus A gene indicated that the ch itinase promoter is activated during attack by the fungal pathogens Botrytis cinerea, Rhizoctonia solani, and Sclerotium rolfsii.
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Cytology of infection of 35S‐bean chitinase transgenic canola plants by Rhizoctonia solani: cytochemical aspects of chitin breakdown in vivo
TL;DR: Reduction in fungal biomass, increase in hyphal alterations leading to fungal lysis and chitin breakdown appear to be typical features observed in transgenic canola plants, likely responsible for the enhanced protection against fungal attack observed in these plants.