Pierre Moënne-Loccoz

TL;DR: This paper aims to demonstrate the efforts towards in-situ applicability of EMMARM, which aims to provide real-time information about the “building blocks” of EMT and its role in disease.

TL;DR: The first definitive evidence for a bridging, symmetrical peroxo adduct is presented from vibrational spectroscopic studies of the freeze-trapped intermediate of this mutant R2.

TL;DR: To characterize this transient intermediate and establish unambiguously the peroxodiferric assignment, rapid freeze-quenching was used to trap the initial intermediate for resonance Raman investigation, andrete vibrational modes are observed for this intermediate, indicating a single chromophore in a homogeneous state, in agreement with the Mössbauer conclusions.

TL;DR: These experiments confirm that the thiolate ligand facilitates the O--O bond cleavage by P450 enzymes and also demonstrate that this ligand satisfies important roles in protein folding, substrate binding, and electron transfer.

TL;DR: Conversion of heme to verdoheme by heme oxygenase-1 (HO-1) is thought to involve α-meso-hydroxylation and elimination of the meso-carbon as CO, a reaction supported by both H2O2 and NADPH-cytochrome P450 reductase/O2.