Poonam Srivastava

TL;DR: The results suggest that vanadate, an insulin-mimetic agent, effectively normalized hyperglycemia, but unlike insulin, could not completely restore the altered endogenous defence mechanisms in diabetic liver.

TL;DR: This work covalently link an alkyne-substituted dirhodium catalyst to a prolyl oligopeptidase containing a genetically encoded L-4-azidophenylalanine residue to create an ArM that catalyses olefin cyclopropanation that reduces the formation of byproducts, including those resulting from the reaction of Dirhodium–carbene intermediates with water.

TL;DR: Overexpression of rhIFN-alpha2b was obtained by synthesizing a codon optimized gene for IFN- alpha2b and expressing it in the form of inclusion bodies (IBs) in Escherichia coli to give a final product yield of approximately 3g/L, which is maximum reported in the literature.

TL;DR: An efficient platform to create and screen libraries of artificial metalloenzymes via random mutagenesis, which is used to evolve highly selective dirhodium cyclopropanases and demonstrates that ArMs evolved for one reaction can serve as starting points to evolve catalysts for others.

TL;DR: The scope of this method with respect to both the scaffold and cofactor components is demonstrated and it is established that the dirhodium ArMs generated can catalyze the decomposition of diazo compounds and both SiH and olefin insertion reactions involving these carbene precursors.