Sensitive optical biosensors for unlabeled targets: a review.

The realization of miniaturized optofluidic platforms offers potential for achieving more functional and more compact devices. Such integrated systems bring fluid and light together and exploit their microscale interaction for a large variety of applications. The high sensitivity of compact microphotonic devices can generate effective microfluidic sensors, with integration capabilities. By turning the technology around, the exploitation of fluid properties holds the promise of highly flexible, tunable or reconfigurable microphotonic devices. We overview some of the exciting developments so far.

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Integrated optofluidics: A new river of light

Refractive index (RI) sensors based on optical resonance techniques are receiving a high degree of attention because of the need to develop simple, low-cost, high-throughput detection technologies for a number of applications. While the sensing mechanism of most of the reported RI sensors is similar, the construction is quite different from technique to technique. It is desirable to have a uniform mechanism for comparing the various RI sensing techniques, but to date there exists a degree of variation as to how the sensing performance is quantified. Here we set forth a rigorous definition for the detection limit of resonant RI sensors that accounts for all parameters that affect the detection performance. Our work will enable a standard approach for quantifying and comparing the performance of optical resonance-based RI sensors. Additionally, it will lead to design strategies for performance improvement of RI sensors.

On the performance quantification of resonant refractive index sensors

Optofluidics - the synergistic integration of photonics and microfluidics - has recently emerged as a new analytical field that provides a number of unique characteristics for enhanced sensing performance and simplification of microsystems. In this review, we describe various optofluidic architectures developed in the past five years, emphasize the mechanisms by which optofluidics enhances bio/chemical analysis capabilities, including sensing and the precise control of biological micro/nanoparticles, and envision new research directions to which optofluidics leads.

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Optofluidic microsystems for chemical and biological analysis

This Review covers photonic crystals (PhCs) and their use for sensing mainly chemical and biochemical parameters, with a particular focus on the materials applied. Specific sections are devoted to a) a lead-in into natural and synthetic photonic nanoarchitectures, b) the various kinds of structures of PhCs, c) reflection and diffraction in PhCs, d) aspects of sensing based on mechanical, thermal, optical, electrical, magnetic, and purely chemical stimuli, e) aspects of biosensing based on biomolecules incorporated into PhCs, and f) current trends and limitations of such sensors.

Photonic Crystals for Chemical Sensing and Biosensing

We demonstrate evanescent-wave sensing of Cy5-DNA-molecules in an aqueous solution using a photonic crystal fiber. Less than 0.8µL sample volume placed in the holes of the fiber is sufficient for reliable detection.

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Photonic crystal fiber based evanescent-wave sensor for detection of biomolecules in aqueous solutions

We present experimental results showing that long-period gratings in photonic crystal fibers can be used as sensitive biochemical sensors. A layer of biomolecules was immobilized on the sides of the holes of the photonic crystal fiber and by observing the shift in the resonant wavelength of a long-period grating it was possible to measure the thickness of the layer. The long-period gratings were inscribed in a large-mode area silica photonic crystal fiber with a CO2 laser. The thicknesses of a monolayer of poly-L-lysine and double-stranded DNA was measured using the device. We find that the grating has a sensitivity of approximately 1.4nm/1nm in terms of the shift in resonance wavelength in nm per nm thickness of biomolecule layer.

https://orbit.dtu.dk/files/10711921/4.pdf

Photonic crystal fiber long-period gratings for biochemical sensing.

We demonstrate highly efficient evanescent-wave detection of fluorophore-labeled biomolecules in aqueous solutions positioned in the air holes of the microstructured part of a photonic crystal fiber. The air-suspended silica structures located between three neighboring air holes in the cladding crystal guide light with a large fraction of the optical field penetrating into the sample even at wavelengths in the visible range. An effective interaction length of several centimeters is obtained when a sample volume of less than 1 µL is used.

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We demonstrate selective detection of fluorophore labeled antibodies from minute samples probed by a sensor layer of complementary biomolecules immobilized inside the air holes of microstructured Polymer Optical Fiber (mPOF). The fiber core is defined by a ring of 6 air holes and a simple procedure was applied to selectively capture either α-streptavidin or α-CRP antibodies inside these air holes. A sensitive and easy-to-use fluorescence method was used for the optical detection. Our results show that mPOF based biosensors can provide reliable and selective antibody detection in ultra small sample volumes.

Selective detection of antibodies in microstructured polymer optical fibers

We present what is believed to be the first microstructured polymer optical fiber (mPOF) fabricated from Topas cyclic olefin copolymer, which has attractive material and biochemical properties. This polymer allows for a novel type of fiber-optic biosensor, where localized sensor layers may be activated on the inner side of the air holes in a predetermined section of the mPOF. The concept is demonstrated using a fluorescence-based method for selective detection of fluorophore-labeled antibodies.

Poul E. Hoiby

Papers

Photonic crystal fiber based evanescent-wave sensor for detection of biomolecules in aqueous solutions

Photonic crystal fiber long-period gratings for biochemical sensing.

Photonic crystal fiber based evanescent-wave sensor for detection of biomolecules in aqueous solutions

Selective detection of antibodies in microstructured polymer optical fibers

Localized biosensing with Topas microstructured polymer optical fiber