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Showing papers in "Optics Express in 2005"


Journal ArticleDOI
TL;DR: Using a high-efficiency grating interferometer for hard X rays (10-30 keV) and a phase-stepping technique, separate radiographs of the phase and absorption profiles of bulk samples can be obtained from a single set of measurements.
Abstract: Using a high-efficiency grating interferometer for hard X rays (10-30 keV) and a phase-stepping technique, separate radiographs of the phase and absorption profiles of bulk samples can be obtained from a single set of measurements. Tomographic reconstruction yields quantitative three-dimensional maps of the X-ray refractive index, with a spatial resolution down to a few microns. The method is mechanically robust, requires little spatial coherence and monochromaticity, and can be scaled up to large fields of view, with a detector of correspondingly moderate spatial resolution. These are important prerequisites for use with laboratory X-ray sources.

1,264 citations


Journal ArticleDOI
TL;DR: This work proposes that the ultimate limit to the attenuation of hollow-core photonic crystal fibres is determined by surface roughness due to frozenin capillary waves, and confirms the wavelength dependence of the minimum loss of fibres drawn to different scales.
Abstract: Hollow-core photonic crystal fibres have excited interest as potential ultra-low loss telecommunications fibres because light propagates mainly in air instead of solid glass. We propose that the ultimate limit to the attenuation of such fibres is determined by surface roughness due to frozenin capillary waves. This is confirmed by measurements of the surface roughness in a HC-PCF, the angular distribution of the power scattered out of the core, and the wavelength dependence of the minimum loss of fibres drawn to different scales.

780 citations


Journal ArticleDOI
TL;DR: High-repetition rate femtosecond lasers are shown to drive heat accumulation processes that are attractive for rapid writing of low-loss optical waveguides in transparent glasses and accurately tracks the waveguide diameter as cumulative heating expands the modification zone above 200-kHz repetition rate.
Abstract: High-repetition rate femtosecond lasers are shown to drive heat accumulation processes that are attractive for rapid writing of low-loss optical waveguides in transparent glasses. A novel femtosecond fiber laser system (IMRA America, FCPA muJewel) providing variable repetition rate between 0.1 and 5 MHz was used to study the relationship between heat accumulation and resulting waveguide properties in fused silica and various borosilicate glasses. Increasing repetition rate was seen to increase the waveguide diameter and decrease the waveguide loss, with waveguides written with 1-MHz repetition rate yielding ~0.2-dB/cm propagation loss in Schott AF45 glass. A finite-difference thermal diffusion model accurately tracks the waveguide diameter as cumulative heating expands the modification zone above 200-kHz repetition rate.

732 citations


Journal ArticleDOI
TL;DR: A digital holographic microscope adapted to the quantitative study of cellular dynamics, in a transmission mode, and allows to determine independently the thickness and the integral refractive index of cells.
Abstract: We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells in culture are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to lambda/1800, and the low acquisition time (~20micros) enable to monitor cellular dynamics processes. An experimental procedure allowing to calculate both the integral refractive index and the cellular thickness (morphometry) from the measured phase shift is presented. Specifically, the method has been applied to study the dynamics of neurons in culture during a hypotonic stress. Such stress produces a paradoxical decrease of the phase which can be entirely resolved by applying the methodological approach described in this article; indeed the method allows to determine independently the thickness and the integral refractive index of cells.

652 citations


Journal ArticleDOI
TL;DR: Techniques of digital holography are improved in order to obtain high-resolution, high-fidelity images of quantitative phase-contrast microscopy, and the angular spectrum method of calculating holographic optical field is seen to have significant advantages including tight control of spurious noise components.
Abstract: Techniques of digital holography are improved in order to obtain high-resolution, high-fidelity images of quantitative phase-contrast microscopy. In particular, the angular spectrum method of calculating holographic optical field is seen to have significant advantages including tight control of spurious noise components. Holographic phase images are obtained with 0.5 μm diffraction-limited lateral resolution and largely immune from the coherent noise common in other holographic techniques. The phase profile is accurate to about 30 nm of optical thickness. Images of SKOV-3 ovarian cancer cells display intracellular and intranuclear organelles with clarity and quantitative accuracy.

651 citations


Journal ArticleDOI
TL;DR: A technique for polarization sensitive optical frequency domain reflectometry (OFDR) that achieves 22 micrometer two-point spatial resolution over 35 meters of optical length with -97 dB sensitivity in a single measurement taking only seconds is described.
Abstract: We describe a technique for polarization sensitive optical frequency domain reflectometry (OFDR) that achieves 22 micrometer two-point spatial resolution over 35 meters of optical length with -97 dB sensitivity in a single measurement taking only seconds. We demonstrate OFDR’s versatility in both time- and frequency-domain metrology by analyzing a fiber Bragg grating (FBG) in both the spectral and impulse response domains. We also demonstrate how a polarization diversity receiver can be used in an OFDR system to track changes in the polarization state of light propagating through a birefringent component.

642 citations


Journal ArticleDOI
TL;DR: In this paper, a high-speed, frequency swept, 1300 nm laser source for frequency domain reflectometry and OCT with Fourier domain/swept source detection is presented, which uses a fiber coupled, semiconductor amplifier and a tunable fiber Fabry-Perot filter.
Abstract: We demonstrate a high-speed, frequency swept, 1300 nm laser source for frequency domain reflectometry and OCT with Fourier domain/swept-source detection. The laser uses a fiber coupled, semiconductor amplifier and a tunable fiber Fabry-Perot filter. We present scaling principles which predict the maximum frequency sweep speed and trade offs in output power, noise and instantaneous linewidth performance. The use of an amplification stage for increasing output power and for spectral shaping is discussed in detail. The laser generates ~45 mW instantaneous peak power at 20 kHz sweep rates with a tuning range of ~120 nm full width. In frequency domain reflectometry and OCT applications the frequency swept laser achieves 108 dB sensitivity and ~10 mum axial resolution in tissue. We also present a fast algorithm for real time calibration of the fringe signal to equally spaced sampling in frequency for high speed OCT image preview.

634 citations


Journal ArticleDOI
TL;DR: This paper demonstrates experimentally that it is possible to control optically the group velocity of an optical pulse as it travels along an optical fiber and derives the basic theory behind these group-delay changes and demonstrates the effect in two kinds of fibers which are conventionally used.
Abstract: We demonstrate experimentally that it is possible to control optically the group velocity of an optical pulse as it travels along an optical fiber. To achieve this control we use the effect of Stimulated Brillouin Scattering. In our experiments we have achieved changes in the group index of 10-3 in several kilometer-length fibers, thus leading to pulse delaying and advancement in the range of tens of nanoseconds. We believe that this is the first evidence of such optically-controlled strong delay changes in optical fibers. In this paper we derive the basic theory behind these group-delay changes and we demonstrate the effect in two kinds of fibers which are conventionally used.

553 citations


Journal ArticleDOI
TL;DR: A silicon modulator with an intrinsic bandwidth of 10 GHz and data transmission from 6 Gbps to 10 Gbps is demonstrated.
Abstract: We demonstrate a silicon modulator with an intrinsic bandwidth of 10 GHz and data transmission from 6 Gbps to 10 Gbps Such unprecedented bandwidth performance in silicon is achieved through improvements in material quality, device design, and driver circuitry

545 citations


Journal ArticleDOI
TL;DR: Measurements indicate that optical loss in these high-Q microresonators is limited not by surface roughness, but rather by surface state absorption and bulk free-carrier absorption.
Abstract: Using a combination of resist reflow to form a highly circular etch mask pattern and a low-damage plasma dry etch, high-quality-factor silicon optical microdisk resonators are fabricated out of silicon-on-insulator (SOI) wafers. Quality factors as high as Q = 5×10^6 are measured in these microresonators, corresponding to a propagation loss coefficient as small as α ~ 0.1 dB/cm. The different optical loss mechanisms are identified through a study of the total optical loss, mode coupling, and thermally-induced optical bistability as a function of microdisk radius (5-30 µm). These measurements indicate that optical loss in these high-Q microresonators is limited not by surface roughness, but rather by surface state absorption and bulk free-carrier absorption.

524 citations


Journal ArticleDOI
TL;DR: A photonic nanocavity with a high Q factor of 100,000 and a modal volume V of 0.71 cubic wavelengths, is demonstrated and a point-defect cavity in a two-dimensional (2D) photonic crystal (PC) slab is improved where the arrangement of six air holes near the cavity edges is fine-tuned.
Abstract: A photonic nanocavity with a high Q factor of 100,000 and a modal volume V of 0.71 cubic wavelengths, is demonstrated. According to the cavity design rule that we discovered recently, we further improve a point-defect cavity in a two-dimensional (2D) photonic crystal (PC) slab, where the arrangement of six air holes near the cavity edges is fine-tuned. We demonstrate that the measured Q factor for the designed cavity increases by a factor of 20 relative to that for a cavity without displaced air holes, while the calculated modal volume remains almost constant.

Journal ArticleDOI
TL;DR: A novel surface plasmon waveguide structure is proposed for highly integrated planar lightwave circuits by etching a small trench through a metallic thin film on a silica substrate, a guided mode with highly confined light fields is realized.
Abstract: A novel surface plasmon waveguide structure is proposed for highly integrated planar lightwave circuits. By etching a small trench through a metallic thin film on a silica substrate, a guided mode with highly confined light fields is realized. The mode properties of the proposed structure are studied. The necessity of using a polymer upper-cladding is discussed. The coupling between two closely positioned waveguides and a 90o bending are also studied numerically. Sharp bending and high integration can be realized with the present surface plasmon waveguide. The proposed structure is easy to fabricate as compared with some other types of surface plasmon waveguides for high integration.

Journal ArticleDOI
TL;DR: The ability of the system to image pulsatile flow in the dermis and to perform functional imaging of large volumes demonstrates the clinical potential of multifunctional spectral-domain OCT.
Abstract: We demonstrate a high-speed multi-functional spectral-domain optical coherence tomography system, using a broadband light source centered at 1.3 µm and two InGaAs line scan cameras capable of acquiring individual axial scans in 24.4 µs, at a rate of 18,500 axial scans per second. Fundamental limitations on the accuracy of phase determination as functions of signal-to-noise ratio and lateral scan speed are presented and their relative contributions are compared. The consequences of phase accuracy are discussed for both Doppler and polarization-sensitive OCT measurements. A birefringence artifact and a calibration procedure to remove this artifact are explained. Images of a chicken breast tissue sample acquired with the system were compared to those taken with a time-domain OCT system for birefringence measurement verification. The ability of the system to image pulsatile flow in the dermis and to perform functional imaging of large volumes demonstrates the clinical potential of multi-functional spectral-domain OCT.

Journal ArticleDOI
TL;DR: Experimental confirmation that super-resolution imaging can be achieved using a 50-nm thick planar silver layer as a near-field lens at wavelengths around 365 nm agrees well with finite-difference time domain (FDTD) simulations.
Abstract: It has been proposed that a planar silver layer could be used to project a super-resolution image in the near field when illuminated near its plasma frequency [J. B. Pendry, Phys. Rev. Lett. 86, 3966 (2000)]. This has been investigated experimentally using a modified form of conformal-mask photolithography, where dielectric spacers and silver layers are coated onto a tungsten-on-glass mask. We report here on the experimental confirmation that super-resolution imaging can be achieved using a 50-nm thick planar silver layer as a near-field lens at wavelengths around 365 nm. Gratings with periods down to 145 nm have been resolved, which agrees well with our finite-difference time domain (FDTD) simulations.

Journal ArticleDOI
TL;DR: Extensive 3-D finite-difference time-domain simulations are carried out to elucidate the nature of surface plasmon polaritons (SPPs) and localized surface plasmons (LSPs) generated by nanoscale holes in thin metallic films interacting with light.
Abstract: Extensive 3-D finite-difference time-domain simulations are carried out to elucidate the nature of surface plasmon polaritons (SPPs) and localized surface plasmon polaritons (LSPs) generated by nanoscale holes in thin metallic films interacting with light. Both isolated nanoholes and square arrays of nanoholes in gold films are considered. For isolated nanoholes, we expand on an earlier discussion of Yin et al. [Appl. Phys. Lett. 85, 467-469 (2004)] on the origins of fringe patterns in the film and the role of nearfield scanning optical microscope probe interactions. The associated light transmission of a single nanohole is enhanced when a LSP excitation of the nanohole itself is excited. Periodic arrays of nanoholes exhibit more complex behavior, with light transmission peaks exhibiting distinct minima and maxima that can be very well described with Fano lineshape models. This behavior is correlated with the coupling of SPP Bloch waves and more directly transmitted waves through the holes.

Journal ArticleDOI
TL;DR: All-optical bistable switching operation of resonant-tunnelling devices with ultra-small high-Q Si photonic-crystal nanocavities with potentials to function as various signal processing functions in photonic andcrystal-based optical-circuits are demonstrated.
Abstract: We have demonstrated all-optical bistable switching operation of resonant-tunnelling devices with ultra-small high-Q Si photonic-crystal nanocavities. Due to their high Q/V ratio, the switching energy is extremely small in comparison with that of conventional devices using the same optical nonlinear mechanism. We also show that they exhibit all-opticaltransistor action by using two resonant modes. These ultrasmall unique nonlinear bistable devices have potentials to function as various signal processing functions in photonic-crystal-based optical-circuits.

Journal ArticleDOI
TL;DR: By introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain full-frame depth resolved imaging completely without scanning, based on temporal focusing of the illumination pulse.
Abstract: The ability to perform optical sectioning is one of the great advantages of laser-scanning microscopy. This introduces, however, a number of difficulties due to the scanning process, such as lower frame rates due to the serial acquisition process. Here we show that by introducing spatiotemporal pulse shaping techniques to multiphoton microscopy it is possible to obtain full-frame depth resolved imaging completely without scanning. Our method relies on temporal focusing of the illumination pulse. The pulsed excitation field is compressed as it propagates through the sample, reaching its shortest duration at the focal plane, before stretching again beyond it. This method is applied to obtain depth-resolved two-photon excitation fluorescence (TPEF) images of drosophila egg-chambers with nearly 105 effective pixels using a standard Ti:Sapphire laser oscillator.

Journal ArticleDOI
TL;DR: It is demonstrated that by correcting for spurious timing-induced phase errors, excellent flow sensitivity can be achieved, limited only by the imaging signal-to-noise ratio.
Abstract: Phase-resolved Doppler optical coherence tomography has been used to image blood flow dynamics in various tissues using both time-domain and spectral-domain optical coherence tomography techniques. In this manuscript, we present phase-resolved Doppler imaging with a high-speed optical frequency domain imaging system. We demonstrate that by correcting for spurious timing-induced phase errors, excellent flow sensitivity can be achieved, limited only by the imaging signal-to-noise ratio. Conventional and Doppler images showing flow in an Intralipid phantom and in human skin are presented. Additionally, we demonstrate the ability of phase-resolved OFDI to measure high flow rates without the deleterious effects of fringe washout.

Journal ArticleDOI
TL;DR: A closed-loop adaptive optics system using a Hartmann-Shack wavefront sensor and a bimorph deformable mirror is combined with Fourier-domain optical coherence tomography to image microscopic blood vessels and the cone photoreceptor mosaic.
Abstract: We have combined Fourier-domain optical coherence tomography (FD-OCT) with a closed-loop adaptive optics (AO) system using a Hartmann-Shack wavefront sensor and a bimorph deformable mirror. The adaptive optics system measures and corrects the wavefront aberration of the human eye for improved lateral resolution (~4 μm) of retinal images, while maintaining the high axial resolution (~6 μm) of stand alone OCT. The AO-OCT instrument enables the three-dimensional (3D) visualization of different retinal structures in vivo with high 3D resolution (4×4×6 μm). Using this system, we have demonstrated the ability to image microscopic blood vessels and the cone photoreceptor mosaic.

Journal ArticleDOI
TL;DR: The demonstration of a terahertz quantum-cascade laser that operates up to 164 K in pulsed mode and 117 K in continuous-wave mode at approximately 3.0 THz is reported.
Abstract: We report the demonstration of a terahertz quantum-cascade laser that operates up to 164 K in pulsed mode and 117 K in continuous-wave mode at approximately 3.0 THz. The active region was based on a resonant-phonon depopulation scheme and a metal-metal waveguide was used for modal confinement. Copper to copper thermocompression wafer bonding was used to fabricate the waveguide, which displayed improved thermal properties compared to a previous indium-gold bonding method.

Journal ArticleDOI
TL;DR: A technique is demonstrated which efficiently transfers light between a tapered standard single-mode optical fiber and a high-Q, ultra-small mode volume, silicon photonic crystal resonant cavity, using this efficient cavity input and output channel to study the steady-state nonlinear absorption and dispersion of the photonics crystal cavity.
Abstract: A technique is demonstrated which efficiently transfers light between a tapered standard single-mode optical fiber and a high-Q, ultra-small mode volume, silicon photonic crystal resonant cavity. Cavity mode quality factors of 4.7×104 are measured, and a total fiber-to-cavity coupling efficiency of 44% is demonstrated. Using this efficient cavity input and output channel, the steady-state nonlinear absorption and dispersion of the photonic crystal cavity is studied. Optical bistability is observed for fiber input powers as low as 250 µW, corresponding to a dropped power of 100 µW and 3 fJ of stored cavity energy. A high-density effective free-carrier lifetime for these silicon photonic crystal resonators of ~ 0.5 ns is also estimated from power dependent loss and dispersion measurements.

Journal ArticleDOI
TL;DR: The observation of four-wave mixing phenomenon in a simple silicon wire waveguide at the optical powers normally employed in communications systems and the wavelength conversion for data rate of 10-Gbps using a 5.8-cm-long silicon wire is reported.
Abstract: We report the observation of four-wave mixing phenomenon in a simple silicon wire waveguide at the optical powers normally employed in communications systems. The maximum conversion efficiency is about -35 dB in the case of a 1.58-cm-long silicon wire waveguide. The nonlinear refractive index coefficient is found to be 9×10-18 m2/W. This value is not negligible for dense wavelength division multiplexing components, because it predicts the possibility of large crosstalk. On the other hand, with longer waveguide lengths with smaller propagation loss, it would be possible to utilize just a simple silicon wire for practical wavelength conversion. We demonstrate the wavelength conversion for data rate of 10-Gbps using a 5.8-cm-long silicon wire. These characteristics are attributed to the extremely small core of silicon wire waveguides.

Journal ArticleDOI
TL;DR: In this paper, the authors study and demonstrate the technique of simultaneous spatial and temporal focusing of femtosecond pulses, with the aim of improving the signal-to-background ratio in multiphoton imaging.
Abstract: We study and demonstrate the technique of simultaneous spatial and temporal focusing of femtosecond pulses, with the aim to improve the signal-to-background ratio in multiphoton imaging. This concept is realized by spatially separating spectral components of pulses into a "rainbow beam" and recombining these components only at the spatial focus of the objective lens. Thus, temporal pulse width becomes a function of distance, with the shortest pulse width confined to the spatial focus. We developed analytical expressions to describe this method and experimentally demonstrated the feasibility. The concept of simultaneous spatial and temporal focusing of femtosecond pulses has the great potential to significantly reduce the background excitation in multiphoton microscopy, which fundamentally limits the imaging depth in highly scattering biological specimens.

Journal ArticleDOI
TL;DR: Experimental results indicate that the proposed novel approach has good performance in speckle noise removal, enhancement and segmentation of the various cellular layers of the retina using the STRATUSOCTTM system.
Abstract: Segmentation of retinal layers from OCT images is fundamental to diagnose the progress of retinal diseases. In this study we show that the retinal layers can be automatically and/or interactively located with good accuracy with the aid of local coherence information of the retinal structure. OCT images are processed using the ideas of texture analysis by means of the structure tensor combined with complex diffusion filtering. Experimental results indicate that our proposed novel approach has good performance in speckle noise removal, enhancement and segmentation of the various cellular layers of the retina using the STRATUSOCT™ system.

Journal ArticleDOI
TL;DR: Numerical simulation of an illustrative lens design example is performed through finite-difference time-domain (FDTD) method and shows agreement with theory analysis result and extraordinary optical transmission of SPPs through sub-wavelength metallic slits is observed in the simulation and helps to improve elements' energy using factor.
Abstract: A novel method is proposed to manipulate beam by modulating light phase through a metallic film with arrayed nano-slits, which have constant depth but variant widths. The slits transport electro-magnetic energy in the form of surface plasmon polaritons (SPPs) in nanometric waveguides and provide desired phase retardations of beam manipulating with variant phase propagation constant. Numerical simulation of an illustrative lens design example is performed through finite-difference time-domain (FDTD) method and shows agreement with theory analysis result. In addition, extraordinary optical transmission of SPPs through sub-wavelength metallic slits is observed in the simulation and helps to improve elements' energy using factor.

Journal ArticleDOI
TL;DR: A simple calibration method using a fringe analysis technique for spectral rescaling is presented, which shows that the system sensitivity is experimentally determined to be 114 dB, and the three-dimensional OCT volumes reveal the structures of the anterior eye segments, which are difficult to observe in two- dimensional OCT images.
Abstract: A two- and three-dimensional swept source optical coherence tomography (SS-OCT) system, which uses a ready-to-ship scanning light source, is demonstrated. The light source has a center wavelength of 1.31 mum, -3 dB wavelength range of 110 nm, scanning rate of 20 KHz, and high linearity in frequency scanning. This paper presents a simple calibration method using a fringe analysis technique for spectral rescaling. This SS-OCT system is capable of realtime display of two-dimensional OCT and can obtain three-dimensional OCT with a measurement time of 2 s. In vivo human anterior eye segments are investigated two- and three-dimensionally. The system sensitivity is experimentally determined to be 114 dB. The three-dimensional OCT volumes reveal the structures of the anterior eye segments, which are difficult to observe in two-dimensional OCT images.

Journal ArticleDOI
TL;DR: This work demonstrates real-time recording of chemical vapor fluc-tuations from 22m away with a fast Fourier-transform infrared (FTIR) spectrometer that uses a laser-like infrared probing beam generated from two 10-fs Ti:sapphire lasers.
Abstract: We demonstrate real-time recording of chemical vapor fluctuations from 22m away with a fast Fourier-transform infrared (FTIR) spectrometer that uses a laser-like infrared probing beam generated from two 10-fs Ti:sapphire lasers. The FTIR’s broad 9–12μm spectrum in the “molecular fingerprint” region is dispersed by fast heterodyne self-scanning, enabling spectra at 2cm-1 resolution to be recorded in 70μs snapshots. We achieve continuous acquisition at a rate of 950 IR spectra per second by actively manipulating the repetition rate of one laser. Potential applications include video-rate chemical imaging and transient spectroscopy of e.g. gas plumes, flames and plasmas, and generally non-repetitive phenomena such as those found in protein folding dynamics and pulsed magnetic fields research.

Journal ArticleDOI
TL;DR: The orbital angular momentum spectra of a light beam can be used to image a variety of intrinsic and extrinsic properties encoded, e.g., in phase and amplitude gradients, dislocations or delays.
Abstract: A major application of optics is imaging all types of structural, physical, chemical and biological features of matter. Techniques based on most known properties of light have been developed over the years to remotely acquire information about such features. They include the spin angular momentum, encoded in the polarization, but not yet the orbital angular momentum encoded in its spiral spectrum. Here we put forward the potential of such spiral spectra. In particular, we use several canonical examples to show how the orbital angular momentum spectra of a light beam can be used to image a variety of intrinsic and extrinsic properties encoded, e.g., in phase and amplitude gradients, dislocations or delays.

Journal ArticleDOI
TL;DR: The deposition of an overlay of higher refractive index than the cladding in a Long Period Fiber Grating permits to improve the sensitivity to ambient refractiveIndex changes in a great manner, and henceforward fast shifts of the resonance wavelength of the attenuations bands in the transmission spectrum are studied.
Abstract: The deposition of an overlay of higher refractive index than the cladding in a Long Period Fiber Grating (LPFG) permits to improve the sensitivity to ambient refractive index changes in a great manner. When the overlay is thick enough, one of the cladding modes is guided by the overlay. This causes important shifts in the effective index values of the cladding modes, and henceforward fast shifts of the resonance wavelength of the attenuations bands in the transmission spectrum. This could be applied for improving the sensitivity of LPFG sensors. The problem is analysed with a numerical method based on LP mode approximation and coupled mode theory, which agrees with so far published experimental results.

Journal ArticleDOI
TL;DR: It is shown that the intermittent fluorescence or 'blinking' of quantum dots can be analyzed by an Independent Component Analysis so as to identify the light emitted by each individual nanoparticle, localize it precisely, and thereby resolve groups of closely spaced (< lambda / 30) quantum dots.
Abstract: In microscopy, single fluorescence point sources can be localized with a precision several times greater than the resolution limit of the microscope. We show that the intermittent fluorescence or 'blinking' of quantum dots can analyzed by an Independent Component Analysis so as to identify the light emitted by each individual nanoparticle, localize it precisely, and thereby resolve groups of closely spaced (< lambda / 30) quantum dots. Both simulated and experimental data demonstrate that this technique is superior to localization based on Maximum Likelihood Estimation of the sum image under the assumption of point emitters. This technique has general application to any emitter with non-Gaussian temporal intensity distribution, including triplet state blinking. When applied to the labeling of structures, a high resolution "image" consisting of individually localized points may be reconstructed leading to the term "Pointillism".