Pradip Raychaudhuri

TL;DR: A function for E1A is demonstrated in mediating a dissociation of transcription factor complexes, allowing new interactions to form and thus changing the transcriptional specificity.

TL;DR: Isolation of a cellular activity can reconstitute the E2F-cyclin-A complex and has permitted a more detailed analysis of the mechanism of E1A dissociation, which finds that sequences in conserved region 1 (CR1) and Conserved region 2 (CR2) are important for dissociation of the E 2F complex, whereas amino-terminal sequences are not required.

TL;DR: An analysis of proteins which bind to the E4 promoter in an attempt to define the basis for E1A control of this gene finds that one of these factors, termed E4F, is increased at least 10‐fold in extracts prepared from Ad5 infected cells and that the increase requires the E 1A gene.

TL;DR: E7 trans activation is functionally related to that mediated by the 12S E1A product, and biochemical studies demonstrate that the E7 protein can alter the interaction of cellular factors with the E2F transcription factor.

TL;DR: The activation process appears to involve a phosphorylation event, because treatment of E4F with alkaline phosphatase abolishes activity and incubation of theosphatase-inactivated factor with an extract from virus infected cells restores activity.