Q. Paula Lei

TL;DR: The potential for high throughput affinity screening of combinatorial libraries using ESI–MS is described based upon the multi-stage MS capability of Fourier transform ion cyclotron resonance instrumentation and involving the characterization of components of the library constituents initially present as complexes with a target biopolymer in the ion trap.

TL;DR: Results indicate that specific interactions between the sulfonamide group on the inhibitor and the Zn(II) ion on CAII were preserved in the gas phase, consistent with the hypothesis that CAII retains, at least partially, the structure of its binding pocket in theGas phase on the time scale (seconds to minutes) of the ESI-FTICR measurements.

TL;DR: In this paper , the authors developed a hydrophobic interaction chromatography (HIC) method to separate and quantify different sulfation proteoforms, which led to the direct functionality assessment of tyrosine-sulfated species.

TL;DR: In this paper , the first known non-affinity purification method for two S constructs, S_dF_2P and HexaPro, expressed in the mammalian cell line, CHO-DG44, was presented.

TL;DR: How analytical characterization tools, especially the microchip reduced capillary gel electrophoresis (CGE-SDS), played a pivotal role in identifying the development issues and in providing measurements to guide implementation of mitigation strategies is detailed.