Qi-Jun Chen

TL;DR: A toolkit that facilitates transient or stable expression of the CRISPR/Cas9 system in a variety of plant species, which will facilitate plant research, as it enables high efficiency generation of mutants bearing multiple gene mutations.

TL;DR: Comparisons of 12 combinations of eight promoters and two terminators found that the efficiency of the egg cell-specific promoter-controlled CRISPR/Cas9 system depended on the presence of a suitable terminator, and the composite promoter generated by fusing two eggcell-specific promoters resulted in much higher efficiency of mutation in the T1 generation compared with the single promoters.

TL;DR: Novel transgene-free herbicide-resistant watermelon varieties were created by base-editing with a potential of immediate field application to facilitate broadleaved weed control in China.

TL;DR: Results demonstrate the OsNHX1 gene was successfully transferred into perennial ryegrass by Agrobacterium-mediated method, and the salt-tolerance of perennial rYegrASS was improved by overexpression of the Os NHX1 genes.

TL;DR: This report constructs a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and Zm ALS2 and achieves much higher prime-editing efficiency in maize.