Showing papers by "Quan Chen published in 2007"
TL;DR: It is demonstrated that the highly effective anticancer agent arsenic induces apoptotic process in yeast cells, and reactive oxygen species (ROS) production was observed in the process and mitochondrial membrane potential decreased after arsenic treatment.
Abstract: In recent years, it has been shown that yeast, a unicellular organism, undergoes apoptosis in response to various factors. Here we demonstrate that the highly effective anticancer agent arsenic induces apoptotic process in yeast cells. Reactive oxygen species (ROS) production was observed in the process. Moreover, mitochondrial membrane potential decreased after arsenic treatment. Resistance of the rho(0) mutant strain (lacking mtDNA) to arsenic provides further evidence that this death process involves mitochondria. In addition, hypersensitivity of Deltasod1 to arsenic suggests the critical role of ROS. Cell death and DNA fragmentation decreased in a Deltayca1 deletion mutant, indicating the participation of yeast caspase-1 protein in apoptosis. The implications of these findings for arsenic-induced apoptosis are discussed.
40 citations
TL;DR: It is proposed that UCP2 may represent an effective weaponry used by germ cells to combat ROS-induced apoptosis and was detected in all germ cells with its level significantly increased if the testes were exposed to 43 degrees C for 5 min.
32 citations