R
Radoje Drmanac
Researcher at Argonne National Laboratory
Publications - 207
Citations - 7906
Radoje Drmanac is an academic researcher from Argonne National Laboratory. The author has contributed to research in topics: Nucleic acid & Sequencing by hybridization. The author has an hindex of 45, co-authored 204 publications receiving 7592 citations.
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Method of sequencing of genomes by hybridization of oligonucleotide probes
TL;DR: In this article, the conditions under which oligonucleotides hybridize only with entirely homologous sequences are recognized by simultaneous hybridization of DNA molecules applied as dots and bound onto a filter.
Patent
Method of sequencing by hybridization of oligonucleotide probes
TL;DR: In this article, the conditions under which oligonucleotide probes hybridize preferentially with entirely complementary and homologous nucleic acid targets are described, using these hybridization conditions, overlapping oligonotide probes associate with a target.
Journal ArticleDOI
Sequencing of megabase plus DNA by hybridization: Theory of the method
TL;DR: Estimates of the types and numbers of oligonucleotides that would have to be synthesized in order to sequence a megabase plus segment of DNA have been used to show advantages over existing methods because of the inherent redundancy and parallelism in its data gathering.
Journal ArticleDOI
DNA sequence determination by hybridization: A strategy for efficient large-scale sequencing
Radoje Drmanac,Snezana Drmanac,Z. Strezoska,Z. Strezoska,Tatjana Paunesku,Ivan Labat,Ivan Labat,M. Zeremski,M. Zeremski,J. Snoddy,W. K. Funkhouser,Ben F. Koop,Ben F. Koop,Leroy Hood,Leroy Hood,R. Crkvenjakov +15 more
TL;DR: The concept of sequencing by hybridization (SBH) makes use of an array of all possible n-nucleotide oligomers (n-mers) to identify n-mers present in an unknown DNA sequence and may accelerate the mapping and sequencing phases of the human genome project.
Journal ArticleDOI
Assessment of the cPAS-based BGISEQ-500 platform for metagenomic sequencing
Chao Fang,Huanzi Zhong,Yuxiang Lin,Bing Chen,Mo Han,Huahui Ren,Haorong Lu,Jacob M. Luber,Min Xia,Wangsheng Li,Shayna Stein,Xun Xu,Wenwei Zhang,Radoje Drmanac,Jian Wang,Huanming Yang,Lennart Hammarström,Aleksandar Kostic,Aleksandar Kostic,Karsten Kristiansen,Junhua Li +20 more
TL;DR: The high accuracy and technical reproducibility confirm the applicability of the new high-throughput sequencing platform BGISEQ-500 for metagenomic studies, though caution is still warranted when combining meetagenomic data from different platforms.