The green synthesis of CuO nanoparticles was synthesized by Allium sativum extract. The powder X-ray diffraction pattern explained the high crystalline nature and the calculated crystallite size in 25−35 nm. The Fourier transform infrared spectrum described the CuO bonding nature. The UV visible analysis reported the surface Plasmon resonance band at 398 nm. The Photoluminescence spectrum denoted the red band emission in the visible region. The particle size analyzer displayed the CuO nanoparticles in a 20−40 nm range of size. The SEM images manifested the spherical, oval-shaped morphology of CuO nanoparticles. The EDAX spectrum and mapping proved the purity of CuO with Cu and O occurrence. The HRTEM images confirmed the crystalline nature formation of CuO nanoparticles. The phytochemical analysis listed the phytochemicals contributed for CuO nanoparticle synthesis via Allium sativum extract. The antimicrobial activity reported the efficient infection on bacteria as well as fungi. The antioxidant activity demonstrated the potential ability of free radical scavenging activity. The potential anti-inflammatory activity against egg albumin has obtained. The anti larvicidal activity showed the notable potential nature of affection on Anopheles subpictus mosquito larvae. At 21st century, CuO nanoparticles from environment friendly synthesis have special efficient applications in drugs, optics, batteries, gas sensors, catalysts, cosmetics, and semiconductor applications. Moreover, CuO nanoparticles have future applications in formulating the drug for human life-threatening diseases and various dangerous cancer treatments.

Green synthesis of CuO nanoparticles via Allium sativum extract and its characterizations on antimicrobial, antioxidant, antilarvicidal activities

Background: Allicin, a major component of garlic, is regarded as a cardioprotective agent and is associated with increased endothelial function. Methods: The effects of allicin on lipopolysaccharide (LPS)-induced vascular oxidative stress and inflammation in cultured human umbilical vein endothelial cells (HUVECs) and the mechanisms underlying these effects were studied. The protective effects were measured using cell viability, a lactate dehydrogenase (LDH) assay and cell apoptosis as indicators, and the anti-oxidative activity was determined by measuring reactive oxygen species (ROS) generation, oxidative products and endogenous antioxidant enzyme activities. HUVEC mitochondrial function was assessed by determining mitochondrial membrane potential (MMP) collapse, cytochrome c production and mitochondrial ATP release. To investigate the potential underlying mechanisms, we also measured the expression of dynamic mitochondrial proteins using western blotting. Furthermore, we evaluated the Nrf2 antioxidant signaling pathway using an enzyme-linked immunosorbent assay (ELISA). Results: Our results demonstrated that allicin enhanced HUVEC proliferation, which was suppressed by LPS exposure, and LDH release. Allicin ameliorated LPS-induced apoptosis, suppressed ROS overproduction, reduced lipid peroxidation and decreased the endogenous antioxidant enzyme activities in HUVECs. These protective effects were associated with the inhibition of mitochondrial dysfunction as indicated by decreases in the MMP collapse, cytochrome c synthesis and mitochondrial ATP release. In addition, allicin attenuated the LPS-induced inflammatory responses, including endothelial cell adhesion and TNF-α and IL-8 production. Furthermore, allicin increased the expression of LXRα in a dose-dependent manner. Allicin-induced attenuation of inflammation was inhibited by LXRα siRNA treatment. Finally, allicin activated NF-E2-related factor 2 (Nrf2), which controls the defense against oxidative stress and inflammation. Conclusions: Taken together, the present data suggest that allicin attenuated the LPS-induced vascular injury process, which may be closely related to the oxidative stress and inflammatory response in HUVECs. Allicin modulated Nrf2 activation and protected the cells against LPS-induced vascular injury. Our findings suggest that allicin attenuated the LPS-induced inflammatory response in blood vessels.

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Allicin Decreases Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Umbilical Vein Endothelial Cells through Suppression of Mitochondrial Dysfunction and Activation of Nrf2.

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Le soin pulpaire chez l’enfant Constance Renaud

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Diplôme d'état de docteur en chirurgie dentaire

Diallyl trisulfide (DATS) protects against apoptosis during myocardial ischemia-reperfusion (MI/R) injury in diabetic state, although the underlying mechanisms remain poorly defined. Previously, we and others demonstrated that silent information regulator 1 (SIRT1) activation inhibited oxidative stress and endoplasmic reticulum (ER) stress during MI/R injury. We hypothesize that DATS reduces diabetic MI/R injury by activating SIRT1 signaling. Streptozotocin (STZ)-induced type 1 diabetic rats were subjected to MI/R surgery with or without perioperative administration of DATS (40 mg/kg). We found that DATS treatment markedly improved left ventricular systolic pressure and the first derivative of left ventricular pressure, reduced myocardial infarct size as well as serum creatine kinase and lactate dehydrogenase activities. Furthermore, the myocardial apoptosis was also suppressed by DATS as evidenced by reduced apoptotic index and cleaved caspase-3 expression. However, these effects were abolished by EX527 (the inhibitor of SIRT1 signaling, 5 mg/kg). We further found that DATS effectively upregulated SIRT1 expression and its nuclear distribution. Additionally, PERK/eIF2α/ATF4/CHOP-mediated ER stress-induced apoptosis was suppressed by DATS treatment. Moreover, DATS significantly activated Nrf-2/HO-1 antioxidant signaling pathway, thus reducing Nox-2/4 expressions. However, the ameliorative effects of DATS on oxidative stress and ER stress-mediated myocardial apoptosis were inhibited by EX527 administration. Taken together, these data suggest that perioperative DATS treatment effectively ameliorates MI/R injury in type 1 diabetic setting by enhancing cardiac SIRT1 signaling. SIRT1 activation not only upregulated Nrf-2/HO-1-mediated antioxidant signaling pathway but also suppressed PERK/eIF2α/ATF4/CHOP-mediated ER stress level, thus reducing myocardial apoptosis and eventually preserving cardiac function.

Diallyl trisulfide ameliorates myocardial ischemia–reperfusion injury by reducing oxidative stress and endoplasmic reticulum stress-mediated apoptosis in type 1 diabetic rats: role of SIRT1 activation

Cysts and tumors associated with impacted third molars: is prophylactic removal justified?

Garlic is one of the components, which have effects on reducing the risk of cancer. Including garlic in the diet helps for the betterment of the health. Medicinal effects of the garlic were known since 5,000 years. Recently, studies were carried out to known its effect on the cancer cell lines. Many studies have shown its effects not only on carcinomas, but also on the cardiovascular system and immune system. Functions of the each component of the garlic were studied to know exactly, which component has got beneficial effect. So this review has been carried out to know about the component, functions of each component, mode of action, and beneficial effects of the garlic.

Garlic: Review of literature.

Objective: To assess the incidence of cystic changes in the impacted lower third molar (ILTM) in which the pericoronal (follicular) space is less than 2.5 mm as measured from the radiograph. The relationship between the cystic changes and patient's age, sex, and angular position and contact of ILTM with adjacent tooth was also evaluated. Materials and Methods: Follicular space less than 2.5 mm as measured from the panoramic radiograph was included in the study. A total of 73 tissue samples collected during the extraction ILTM were examined histopathologically. Then the data were analyzed for associations with age, sex, angular position, and contact of the ILTM with an adjacent tooth. Results: There were 37 male and 36 female patients, age ranging from 17 to 35 years (mean 23.95 years). Out of 73 specimens, 17 (23.3%) showed cystic changes; among them 16 (22.1%) showed dentigerous cysts and 1 (1.2%) showed odontogenic keratocysts. Most of the cystic changes occurred in the 26-30 year age range. The cystic changes showed male predominance but could not gain statistical significance. The relationship between cystic changes and angular position was statistically significant (P Conclusion: Incidence of cystic changes in ILTM justifies extraction of the impacted tooth associated with symptoms. The decision to extract or not to extract impacted third molar should be individualized, rather than generalized.

Incidence of cystic changes in impacted lower third molar.

Phonetics is an important function of oral cavity. It has been overlooked quite frequently while fabricating the complete dentures. In this study modification of anterior palatal surface of denture is done and assessed for its impact on phonetics. Purpose is to assess acoustic and speech intelligibility analysis in edentulous patients and also to evaluate the influence of conventional dentures, arbitrary rugae and customized rugae dentures on speech in complete denture wearers. Ten healthy edentulous patients 55–70 years of age were selected for the study. Dentures were fabricated in conventional way for these patients. Recordings were done for intelligibility and acoustic analysis of the speech. Recordings were done without denture, with conventional denture, with arbitrary rugae denture, with customized rugae denture. Each recording was done at an interval of 10 days period. All four recordings were analyzed and comparison was done using paired ‘t’ test. There was significant improvement in frequency peak noise energy for ‘s’, antiformant frequency for ‘n’ in rugae incorporated dentures. There was relative improvement in frequency peak noise energy for ‘sh’, frequency proximity burst, voice onset time for ‘d’, with rugae incorporated dentures. Findings of intelligibility analysis have shown substitution errors with conventional dentures. There was relative improvement of speech with rugae incorporated dentures. Among these, customized rugae dentures showed better results than arbitrary rugae dentures.

Acoustic Analysis and Speech Intelligibility in Patients Wearing Conventional Dentures and Rugae Incorporated Dentures

Hyperdontia or supernumerary teeth without associated syndromes is a rare phenomenon, as supernumerary teeth are usually associated with cleft lip and palate or other syndromes such as Gardeners syndrome, cleidocranial dysplasia, trichorhinophalangic syndrome, etc. A 15-year-old girl reported for orthodontic treatment with a chief complaint of irregular teeth and unaesthetic smile. On examination class I malocclusion with severe crowding of upper anteriors, anterior open bite of 5 mm, cross bite with upper right posteriors, upper midline shift to right by 5 mm and two supernumerary teeth placed buccally in between 16 & 17 and 26 & 27, respectively, were found. On routine radiographical examination, two additional impacted supernumerary teeth were noticed distal to 18 and in mandibular arch in between roots of 35 and 36. A general physician was consulted who confirmed that there was no associated syndrome. Family history did not reveal any positive findings.

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Non-syndromic multiple supernumerary teeth: a rare entity.

Treating diverse maxillofacial patients poses a challenge to the maxillofacial prosthodontist. Rehabilitation of hemimandibulectomy patients must aim at restoring mastication and other functions such as intelligible speech, swallowing, and esthetics. Prosthetic methods such as palatal ramp and mandibular guiding flange reposition the deviated mandible. Such prosthesis can also be used to restore speech in case of patients with debilitating speech following surgical resection. This clinical report gives detail of a hemimandibulectomy patient provided with an interim removable dental speech prosthesis with composite resin flange for mandibular guidance therapy.

Raghavendra Adaki

Papers

Garlic: Review of literature.

Incidence of cystic changes in impacted lower third molar.

Acoustic Analysis and Speech Intelligibility in Patients Wearing Conventional Dentures and Rugae Incorporated Dentures

Non-syndromic multiple supernumerary teeth: a rare entity.

A novel speech prosthesis for mandibular guidance therapy in hemimandibulectomy patient: A clinical report