Technology development for the production of biobased products from biorefinery carbohydrates—the US Department of Energy’s “Top 10” revisited

介绍了Kirk—Othmer Encyclopedia of Chemical Technology（化工技术百科全书）（第五版）电子图书网络版数据库，并对该数据库使用方法和检索途径作出了说明，且结合实例简单地介绍了该数据库的检索方法。

Kirk-Othmer Encyclopedia of Chemical Technology数据库介绍及实例

Biodegradation aspects of Polycyclic Aromatic Hydrocarbons (PAHs): A review

Industrial applications of microbial lipases

Lyophilization and development of solid protein pharmaceuticals

Biphasic systems formed by mixing of two polymers or a polymer and a salt in water can be used for separation of cells, membranes, viruses, proteins, nucleic acids, and other biomolecules. The partitioning between the two phases is dependent on the surface properties and conformation of the materials, and also on the composition of the two-phase system. The mechanism of partitioning is, however, complex and not easily predicted. Aqueous two-phase systems (ATPS) have proven to be a useful tool for analysis of biomolecular and cellular surfaces and their interactions, fractionation of cell populations, product recovery in biotechnology, and so forth. Potential for environmental remediation has also been suggested. Because ATPS are easily scalable and are also able to hold high biomass load in comparison with other separation techniques, the application that has attracted most interest so far has been the large-scale recovery of proteins from crude feedstocks. As chemicals constitute the major cost factor for large-scale systems, use of easily recyclable phase components and the phase systems generated by a single-phase chemical in water are being studied.

Aqueous two-phase systems. A general overview.

Aqueous Two-Phase Systems: A General Overview, Rajni Hatti-Kaul. Preparation and Characterization of Aqueous Two-Phase Systems: The Phase Diagram, Anita Kaul. Preperation of Aqueous Two-Phase Systems, Daniel Forciniti. Measurement of Some Physical Properties of Aqueous Two-Phase Systems, Donald E. Brooks and Raymond Norris-Jones. Partitioning Techniques: Single-Step Partitioning in Aqueous Two-Phase Systems, Telma Teixeira Franco and Rajni Hatti-Kaul. Partition by Countercurrent Distribution (CCD), Hans-Erik Akerlund. Liquid-Liquid Partition Chromatography (LLPC), Ulla-Britt Hansson and Christer Wingren. Partitioning of Soluble Molecules and Particulates: Metal Ion Separations in Aqueous Biphasic Systems and with ABECTM Resins, Jonathan G. Huddleston, Scott T. Griffin, Jianhua Zhang Heather D. Willauer, and Robin D. Rogers. Partition of Amino Acids and Peptides in Aqueous Two-Phase Systems, I-Ming Chu and Wen-Yi Chen. Predicting Partition Coefficients of Small Solutes Based on Hydrophobicity, Mark A. Eiteman. Eukaryotic Cell Partition: Experimental Considerations, James M. Van Alstine. Concentration and Purification of Viruses, Lena Hammar. Isolation of Plant Plasma Membranes and Production of Inside-Out Vesicles, Christer Larsson and Susanne Widell. Isolation of Inside-Out Thylakoid Vesicles, Hans-Erik Akerlund. Two-Phase Partitioning as a Method for Isolation of Tight Plasma Membrane Vesicles from Saccharomyces cerevisiae and from Chlamydomonas reinhardtii, Birgitta Norling. Purification of Cyanobacterial Thylakoid, Plasma, and Outer Membranes by Two-Phase Partitioning, Birgitta Norling. Purification of Plasma Membranes by Affinity Partitioning, Bengt Jergil and Lars Ekblad. Studying the Influence of Salts on Partitioning of Proteins: Isoelectric Point Determination, Daniel Forciniti. Partitioning of Chemically Modified Proteins: A Method for Detection of the Resolution of Aqueous Two-Phase Systems, Telma Teixeira Franco. Detection and Analysis of Interactionsby Two-Phase Partition, Lars Backman. Cryopartitioning in Two-Phase Systems Containing Organic Solvents, Gote Johansson. Isolation and Purification of proteins by Two-Phase Ectraction: Optimization of Extractions in Aqueous Two-Phase Systems, Ulrich Menge. Two-Phase Extraction of Proteins from Cell Debris, Kristina Kohler Van Alstine and Andres Veide. Aqueous Two-Phase Extraction of Proteins from Animal Tissue, Michael J. Boland. Chaotropic Aqueous Two-Phase Systems: Preparation and Uses, Daniel Forciniti. Temperature-Induced Phase Partitioning for Protein Purification, Anita Kaul, Josefine Persson, and Folke Tjerneld. Extraction of Amphiphilic Proteins Using Detergent-Based Aqueous Two-Phase Systems, Torsten Minuth. Use of Charged PEG and Dextran Derivatives for Biomolecule Partitioning, Gote Johansson. Affinity Partitioning Using Poly(ethylene glycol) with Covalently Coupled Hydrophobic Groups, Vithaldas P. Shanbhag and Poul Erik H. Jensen. Dye-Ligand Affinity Partitioning of Proteins, Gerhard Kopperschlager and Jurgen Kirchberger. Metal Affinity Protein Partitioning, Roberto Z. Guzman and Javier E. Garcia. Recovery of Proteins and Phase Components, Gote Johansson. Combination of Extraction with Adsorption for Protein Purification, Gabriel Raya-Tonetti and Nora I. Perotti. Integration of Extraction with Affinity Precipitation, Masamichi Kamihira, Rajni Hatti-Kaul, and Bo Mattiasson. Affinity Partitioning Using Magnetic Two-Phase Systems, Masamichi Kamihira. Large-Scale Extraction of Proteins, Teresa Cunha and Raquel Aires-Barros. Bioconversions in Two-Phase Systems: Extractive Bioconversion in Aqueous Two-Phase Systems, Rajni Hatti-Kaul. Enzyme Reaction in Polymer-Polymer Organic Solvent Two-Phase System, Bo Mattiasson. Index.

Aqueous Two-Phase Systems : Methods and Protocols

Industrial biotechnology for the production of bio-based chemicals – a cradle-to-grave perspective

Purification and characterization of cellulases produced by two Bacillus strains.

Biodegradable materials with plastic or elastomeric properties are in great demand for a variety of applications. Polyhydroxyalkanoates (PHAs), polyesters synthesized by microorganisms, possess such desired features. Industrial production of PHAs is currently achieved using recombinant Escherichia coli. Nevertheless, recent research on halophiles, salt requiring microorganisms, has shown a remarkable potential for biotechnological production of PHAs. The halophilic archaeon Haloferax mediterranei accumulates a co-polymer, i.e., poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in large amounts using glucose, starch, and hydrolyzed whey as carbon sources. Chemical composition and molecular weight of PHAs produced by H. mediterranei can be modified depending on the substrate utilized as precursor. Phylogenetic studies on haloarchaeal enzymes able to polymerize the components of PHAs (i.e., PHA synthases) reveal a novel cluster, with a close relationship with PHA polymerases of bacteria and archaea found in marine-related niches. On the other hand, sequences of PHA synthases of two halophilic bacteria are more closely affiliated to synthases of Proteobacteria. Several bacterial species of the family Halomonadaceae accumulate PHAs. Halomonas boliviensis reached PHA yields and volumetric productivities close to the highest reported so far. Furthermore, H. boliviensis and other Halomonas species are able to co-produce PHA and osmolytes, i.e., ectoines and hydroxyectoine, in one process.

Rajni Hatti-Kaul

Papers

Aqueous two-phase systems. A general overview.

Aqueous Two-Phase Systems : Methods and Protocols

Industrial biotechnology for the production of bio-based chemicals – a cradle-to-grave perspective

Purification and characterization of cellulases produced by two Bacillus strains.

Synthesis and production of polyhydroxyalkanoates by halophiles: current potential and future prospects