Showing papers in "Journal of Biotechnology in 2000"

TL;DR: In this article, several aspects, including safety, functional and technological characteristics, have to be taken into consideration in the selection process of probiotic micro-organisms, including origin (healthy human GI tract), non-pathogenicity and antibiotic resistance characteristics.

TL;DR: This review intends to provide an overall picture of the various methods employed for the removal of endotoxins from protein solutions and to optimise a method with regard to the specific properties of endotoxin in aqueous solution.

TL;DR: It is clear from the review that particulate biofilm reactors can be considered a grown up technology for which good design and scale-up guidelines are available.

TL;DR: Results support a hydroxyl radical-generating mechanism whereby the radical can degrade and modify cellulose, hemicellulose and lignin and several other applications have been suggested.

TL;DR: The secondary structure and the thermostability of bovine serum albumin, before adsorption and after homomolecular displacement from silica and polystyrene particles, are studied by circular dichroism spectroscopy and differential scanning calorimetry.

TL;DR: Only sodium azide acted as a true laccase inhibitor and showed no significant interference with the enzyme tests, and the previously reported inhibitory effects result from the reductions of the reaction products such as ABTS radical cation and diquinone or subsequent non-enzymatic interactions during substrate oxidation.

TL;DR: This strain represents a potential source of lutein, a commercially interesting carotenoid of application in aquaculture and poultry farming, as well as in the prevention of cancer and diseases related to retinal degeneration.

TL;DR: Heavy chain immunoglobulins directed against two hapten molecules, the azo-dyes RR6 and RR120 as well as the (proteinaceous) human pregnancy hormone, have been raised in Lama glama and specific VHH fragments for all three antigens are selected by direct screening of Escherichia coli or yeast libraries, even without prior enrichment via bio-panning.

TL;DR: The strain presented a strong tolerance at the inhibitory effect of the 1,3-propanediol, even at high concentrations of this substance at the chemostat, and was associated with cell growth whereas acetate and butyrate seemed non growth-associated products.

TL;DR: Cellulases produced by two Bacillus strains, CH43 and HR68, isolated from hot springs in Zimbabwe, were purified to homogeneity from culture supernatants, showing 100% homology with that of endoglucanases from Bacillus subtilis belonging to glycoside hydrolase family five.

TL;DR: Transgenic tomato plants expressing ACC deaminase, especially those controlled by the PRB-1b promoter, acquired a greater amount of metal within the plant tissues, and were less subject to the deleterious effects of the metals on plant growth than were non-transgenic plants.

TL;DR: Global comparisons of the amino acid compositions and sequences of proteins from mesophiles and extremophiles did not result in general rules of protein stabilization, even after including complete genome sequences into the search.

TL;DR: How DNA microarrays can be applied in the working fields of: safety, functionality and health of food and gene discovery and pathway engineering in plants, and data handling are discussed.

TL;DR: A proteomics approach has been developed aimed to allow high throughput analysis of protein products expressed from cDNA fragments (expressed sequence tags, ESTs), which yielded functional antibodies that gave specific staining in Western blot screening of samples from various cell types.

TL;DR: The mechanism of gel formation seems to be affected by pH, because at pH 7.6, the disulphide bridge between the acidic and the basic polypeptide of glycinin is broken upon heating, and at pH 3.8, notable gel formation did not start upon heat denaturation of beta-conglycinin.

TL;DR: Immobilization of Lentinula edodes lAccase on Eupergit C increased pH, thermal and proteolytic stability with slight modifications in laccase oxidation efficiency and proved to be efficiently stable in removing olive mill wastewater phenolics.

TL;DR: It was apparent that the enzyme was able to remove the 4-O-methylglucuronic groups mainly from soluble substrates from water-soluble polymeric softwood arabinoglucuronoxylans.

TL;DR: High performance liquid chromatography of products from the oxidized models showed that they were produced in sufficient yields to account for the ability of laccase/HOBT to depolymerize nonphenolic lignin.

TL;DR: Although the drainage properties of the pulps could be improved by selected enzymes, the water retention capacity of the dried hornified fibers could not be recovered by any of the enzymes tested.

TL;DR: Fructosyltransferase and invertase have been purified from the crude extract of Aspergillus niger AS0023 by successive chromatographies and the two enzymes exhibited distinctly different kinetic constants confirming their different nature.

TL;DR: A commercially viable diagnostic system incorporating PhotoSELEX-evolved aptamers capable of simultaneous quantification of a large number of analyte molecules is described, which benefits from covalent bonding of aptamer to target protein allowing vigorous washing with denaturants to improve signal to noise.

TL;DR: The cells of Saccharomyces cerevisiae ATCC 24553, were immobilized in k-carrageenan and packed in a tapered glass column reactor for ethanol production from pineapple cannery waste at temperature 30 degrees C and pH 4.5, without any loss in the immobilized cell activity.

TL;DR: Immobilization of highly oriented antibody molecules was realized with the engineered IgG-binding protein and antigen-binding activity of immobilized antibody molecules through the use of assembled B5C1 on a gold surface was about 4.3 times higher than that of physically adsorbed antibody molecules.

TL;DR: Results indicate that the pectinolytic activity of the Amycolata sp.

TL;DR: The ability of the Pichia pastoris-based technology for large-scale production of recombinant hepatitis B virus surface antigen (HBsAg) and both reproducibly purify HBsAg and remove most of the relevant contaminants was ascertained by evaluating ten industrial production batches.

TL;DR: 31P nuclear magnetic resonance (31P NMR) spectra were highly invariable, indicating approximate steady-state intracellular conditions during long term measurements in the filamentous fungus Aspergillus niger.

TL;DR: Green alga Chlamydomonas reinhardtii is not able to maintain a high photosynthetic capacity under medium-duration light/dark cycles and since specific light absorption did not change, light utilization efficiency decreased in comparison to continuous illumination.

TL;DR: These results represent the first report on the application of the recombinant DNA methodology for the obtaining of improved phosphate solubilizing ability from rhizobacterial strains for biofertilization purposes.

TL;DR: The utility of an off-line combination of 400 MHz proton (1H)-NMR spectroscopy and liquid chromatography for the multi-component comparison of low-molecular weight compounds (i.e. chemical fingerprinting) in complex plant matrices is examined.

TL;DR: The NMR-determined solution conformation ofheparin has been used to predict binding sites on the surface of heparin-binding proteins, and the anticoagulant activity of algal fucans has been compared with that of regular, linear sulphated fucan from marine echinoderms.