R
Rasmus Ø. Thorsen
Researcher at Delft University of Technology
Publications - 16
Citations - 260
Rasmus Ø. Thorsen is an academic researcher from Delft University of Technology. The author has contributed to research in topics: Point spread function & Adaptive optics. The author has an hindex of 5, co-authored 15 publications receiving 143 citations. Previous affiliations of Rasmus Ø. Thorsen include Technical University of Denmark.
Papers
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Journal ArticleDOI
Localization microscopy at doubled precision with patterned illumination
Jelmer Cnossen,Taylor Hinsdale,Rasmus Ø. Thorsen,Marijn E. Siemons,Florian Schueder,Florian Schueder,Ralf Jungmann,Ralf Jungmann,Carlas Smith,Carlas Smith,Bernd Rieger,Sjoerd Stallinga +11 more
TL;DR: SIMFLUX combines elements of MINFLUX with structured illumination to double localization precision and improve resolution in localization microscopy.
Journal ArticleDOI
High precision wavefront control in point spread function engineering for single emitter localization
TL;DR: This work presents a calibration and alignment protocol for fluorescence microscopes equipped with a spatial light modulator (SLM) with the goal of establishing a wavefront error well below the diffraction limit for optimum application of complex engineered PSFs.
Journal ArticleDOI
Simultaneous orientation and 3D localization microscopy with a Vortex point spread function.
Christiaan N. Hulleman,Rasmus Ø. Thorsen,Eugene Kim,Cees Dekker,Sjoerd Stallinga,Bernd Rieger +5 more
TL;DR: In this paper, a Vortex point spread function was used to estimate the 3D position, dipole orientation, and degree of rotational constraint from a single 2D image, within 30% of the Cramer-Rao bound.
Posted ContentDOI
Simultaneous orientation and 3D localization microscopy with a Vortex point spread function
TL;DR: An engineered Point Spread Function to enable the simultaneous estimation of dipole orientation, 3D position and degree of rotational constraint of single-molecule emitters from a single 2D focal plane is developed.
Journal ArticleDOI
Impact of optical aberrations on axial position determination by photometry.
Rasmus Ø. Thorsen,Christiaan N. Hulleman,Mathias Hammer,David Grunwald,Sjoerd Stallinga,Bernd Rieger +5 more
TL;DR: It is shown that the photometric ratio as an indicator for axial position is susceptible even to typical optical aberrations, and that Gaussian PSF fitting performed worse than the other methods, as a Gaussian cannot fit the long tail at all.