Showing papers by "Rita Kundu published in 2018"
TL;DR: In this paper, low concentrations of nZVI nanoparticles were evaluated for their growth enhancement potential as seed priming agent in an aromatic rice cultivar, Oryza sativa cv. Gobindabhog.
67 citations
TL;DR: In this article, the essential oils were extracted from seven local varieties of P. betle L. leaves (Bangla, Bagerhati, Manikdanga, Meetha, Kalibangla, Chhaanchi and Ghanagete) with the aim to characterize the varieties on the basis of oil constituents and to study their enzyme inhibitory and cytotoxic properties.
31 citations
TL;DR: The title family of mixed-ligand oxidovanadium(V) hydrazone complexes are [VVO(HL1)(hq)] (1) and [V VO(HL2)(hqs)] (2), where (HL1)2− and (HL2)2+ are the dinegative form of 2-hydroxybenzoylhydrazone o...
Abstract: The title family of mixed-ligand oxidovanadium(V) hydrazone complexes are [VVO(HL1)(hq)] (1) and [VVO(HL2)(hq)] (2), where (HL1)2− and (HL2)2− are the dinegative form of 2-hydroxybenzoylhydrazone o...
31 citations
TL;DR: BA effectively induced DNA damage and apoptosis in SiHa cells and the mechanism of apoptosis was caspase independent and through mitochondrial pathways.
Abstract: Betulinic acid (BA) is a naturally occurring terpenoid found principally in the bark of birch trees as well as in numerous other plants. BA is reported to inhibit cancer progression and induce apoptosis in multiple tumor types. In the present study we have investigated the cytotoxicity and potential genotoxicity of BA in SiHa cells. The cell viability was measured by using MTT assay and the morphological changes, DNA damage, changes in cell cycle and mitochondrial membrane potential (MMP) were used for the assessment of apoptosis. BA was shown to destroy SiHa cells preferentially in a concentration dependent manner with a 50% inhibition of the cells at 39.83 μg/ml. The growth inhibition of the cells by BA was coupled with DNA strand breaks, morphological changes, disruption of MMP, reactive oxygen species (ROS) generation and the cell arrest at G0/G1 stage of cell cycle. BA induced apoptosis in SiHa cells was confirmed by positive Annexin V FITC-PI staining. Our results indicate that BA effectively induced DNA damage and apoptosis in SiHa cells. The mechanism of apoptosis was caspase independent and through mitochondrial pathways.
24 citations
TL;DR: Treatment of 7-day old rice seedlings with 10 μM cadmium chloride resulted in Cd toxicity across the seven non-aromatic and six aromatic rice cultivars and landraces used for the study, which indicated Cd induced tissue damage in plants.
Abstract: Constant exposure of the living ecosystems to heavy metals, like cadmium (Cd), induces a detectable change at the biochemical and genetic level. Repeated application of phosphate fertilizers in paddy fields, leads to increase in Cd content of soil. Cd being highly mobile is transported to shoot and grain, thereby entering into the food chain of animal system. In the present study, treatment of 7-day old rice seedlings with 10 μM cadmium chloride resulted in Cd toxicity across the seven non-aromatic and six aromatic rice cultivars and landraces used for the study. Free proline and malondialdehyde content of treated samples were higher in comparison to the untreated samples, which indicated Cd induced tissue damage in plants. Photosynthetic pigment content of treated samples was also found to be much lower in comparison to the untreated samples, which is probably due to peroxidation of membrane, leading to compromised and lower photosynthetic efficiency of treated plants. At the genetic level, Randomly Amplified Polymorphic DNA assay was found to efficiently detect the genetic polymorphisms (caused by alterations in DNA bases) induced by Cd. Production of unique polymorphic bands in Cd-treated plants helps in assessment of the degree of damage Cd imparts on the plant system. Cluster analysis was performed and the rice genotypes were grouped into five distinct clusters, with IR64 and Tulsibhog in two distinct groups. Based on the variability in responses, the 13 rice genotypes were grouped into sensitive and tolerant ones.
9 citations
TL;DR: ROS mediated DNA damage and induction of apoptosis in cervical cancer cells by Heliotropium indicum L.in ROS mediated DNA damaged cells induces apoptosis, leading to cell death.
Abstract: © 2018 Subhabrata Paul and Rita Kundu. This is an open access article distributed under the terms of the Creative Commons Attribution License -NonCommercialShareAlikeUnported License (http://creativecommons.org/licenses/by-nc-sa/3.0/). *Corresponding Author Rita Kundu, Centre of Advanced Study, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, West Bengal, India. E-mail: kundu_rita @ yahoo.co.in ROS mediated DNA damage and induction of apoptosis in cervical cancer cells by Heliotropium indicum L.
4 citations
TL;DR: It is suggested that the chloroform fraction of the algae was responsible for induction of apoptosis followed by autophagic cell death in SiHa and can serve as a potential candidate for anticancer drug development in future.
Abstract: Objective: Algae is a rich source of various metabolites. Use of algae in traditional medicine was reported from very early days. The potential anticancerous activity of the algae was also reported by recent researches. In the present study, cell death mechanism induced by green alga Chaetomorpha linum ( Muller ) Kutz. was studied on human cervical cancer cell line, SiHa (Human papilloma virus-HPV 16+ve). Methods: Cells were treated with chloroform fraction of the Chaetomorpha linum (CLC). DNA fragmentation, cell proliferation, nuclear morphology, localization of autophagosomes and expression of relevant proteins were studied. Cell cycle and mitochondrial membrane potential (MMP) were assessed by fluorescence assisted cell sorting (FACS). Gas chromatography liquid spectrometry (GCMS) analysis of CLC was done to detect the algal compounds. Results: Preliminary experiments confirmed the absence of DNA fragmentation and altered nuclear morphology in the treated cells. In the Acridine Orange stained cells, the presence of autophagosomes was observed by both microscopy and FACS analysis. Decreased MMP was observed in the treated sets. Wound healing assay showed the inhibitory activity of CLC on SiHa cells. Increased expression of autophagic proteins, viz; LC3BII, p62/SQSTM, Beclin-1 and decreased expression of S6 were observed in the treated sets; indicating that autophagic cell death was induced. Preliminary qualitative chemical study and GCMS analysis detected the presence of saponins, glycosides, alkaloids, phenols, steroids and fatty acids in CLC. Conclusion: All these findings clearly suggested that the chloroform fraction of the algae was responsible for induction of apoptosis followed by autophagic cell death in SiHa and can serve as a potential candidate for anticancer drug development in future.
1 citations