R
Robert F. Silva
Researcher at United States Department of Agriculture
Publications - 70
Citations - 2079
Robert F. Silva is an academic researcher from United States Department of Agriculture. The author has contributed to research in topics: Marek's disease & Virus. The author has an hindex of 24, co-authored 70 publications receiving 1961 citations. Previous affiliations of Robert F. Silva include Agricultural Research Service.
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Marek's disease virus-encoded Meq gene is involved in transformation of lymphocytes but is dispensable for replication
Blanca Lupiani,Lucy F. Lee,Xiaoping Cui,Isabel M. Gimeno,Amy S. Anderson,Robin W. Morgan,Robert F. Silva,Richard L. Witter,Hsing Jien Kung,Sanjay M. Reddy +9 more
TL;DR: In this paper, an rMd5DeltaMeq virus was generated by deleting both copies of the meq gene from the genome of a very virulent strain of MDV.
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Rescue of a pathogenic Marek's disease virus with overlapping cosmid DNAs: Use of a pp38 mutant to validate the technology for the study of gene function
Sanjay M. Reddy,Blanca Lupiani,Isabel M. Gimeno,Robert F. Silva,Lucy F. Lee,Richard L. Witter +5 more
TL;DR: Development of overlapping cosmid DNA clones from a very virulent oncogenic strain of Marek's disease virus resulted in the generation of a recombinant MDV (rMd5) with biological properties similar to the parental strain, to demonstrate the applicability of this technology for elucidation of gene function of MDV.
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Monoclonal antibody-mediated immunoprecipitation of proteins from cell, infected with marek's disease virus or turkey herpesvirus
Robert F. Silva,Lucy F. Lee +1 more
TL;DR: Competition immunoprecipitation and peptide mapping by limited proteolysis confirmed that the three glycoproteins and the 79,000-Da protein contain MDV-HVT common epitopes.
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Comparison of Chinese field strains of avian leukosis subgroup J viruses with prototype strain HPRS-103 and United States strains
TL;DR: Envelope protein gp85 amino acid sequence analysis demonstrated that, interestingly, all recent Chinese isolates were more closely related to HPRS-103 and the earliest U.S. isolates, implying that envelope gp85 may not have diverged from prototype and older strains.
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Hypervariability in the envelope genes of subgroup J avian leukosis viruses obtained from different farms in the United States.
TL;DR: Using the published sequences from HPRS-103, a set of PCR primers that amplified proviral DNA from nine U.S. field isolates suggested that the envelope genes of ALV-J may have arisen as a result of a recombination event between exogenous ALV and Line 0-like sequences in the chicken.