Rolf Rauh

TL;DR: It is demonstrated that the historical SVA SVV 001 strain presents low virulence in pigs when compared to the contemporary SVA SD15-26 strain.

TL;DR: Analysis of porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus (IAV) circulation in growing pigs from three Polish production farms, using Real Time PCR and ELISA testing of oral fluid and serum revealed striking differences in PRRSV detection patterns.

TL;DR: The EZ-SARS-CoV-2 RT-PCR assay performance attributes of high sensitivity, excellent performance in AN sample matrix and in pooled upper respiratory samples support its use in a high-throughput surveillance testing program.

TL;DR: Evaluated measures including sample preparation methods, a dried-down assay, and a portable, battery-powered real-time PCR instrument, to improve molecular diagnosis under field conditions found an inhibition control assay proved to be helpful in avoiding false negative results when interpreting negative results of samples that might be of low quality or with inadequate reduction in inhibitory substances.

TL;DR: The success of VI was inversely related to the dilution of the sample; the assay was generally unsuccessful at lower virus concentrations, and significant differences in reproducibility were observed among the assays evaluated.