R
Rolf Wenderoth
Researcher at Leibniz Association
Publications - 10
Citations - 520
Rolf Wenderoth is an academic researcher from Leibniz Association. The author has contributed to research in topics: Recombinant DNA & Escherichia coli. The author has an hindex of 7, co-authored 10 publications receiving 515 citations. Previous affiliations of Rolf Wenderoth include Merck & Co..
Papers
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Journal ArticleDOI
Improved bivalent miniantibodies, with identical avidity as whole antibodies, produced by high cell density fermentation of Escherichia coli.
Peter Pack,Marian Kujau,Volker Schroeckh,Uwe Knüpfer,Rolf Wenderoth,Dieter Riesenberg,Andreas Plückthun +6 more
TL;DR: A high-cell density fermentation of E. coli producing these miniantibodies is reported and a work-up procedure suitable for large scale production is reported, without any need of subsequent chemical coupling in vitro.
Journal ArticleDOI
High volumetric yields of functional dimeric miniantibodies in Escherichia coli, using an optimized expression vector and high-cell-density fermentation under non-limited growth conditions.
Uwe Horn,Wolfgang Strittmatter,Anke Krebber,Uwe Knüpfer,Marian Kujau,Rolf Wenderoth,Kristian M. Müller,Siegfried Matzku,Andreas Plückthun,Dieter Riesenberg +9 more
TL;DR: Functional bivalent miniantibodies, directed against the epidermal growth factor receptor, accumulated to more than 3 gl−1 in high-cell-density cultures of Escherichia coli RV308(pHKK) on a pilot scale.
Patent
Process for the preparation of recombinant proteins in e.coli by high cell density fermentation
Strittmatter Wolfgang,Matzku Siegfried,Dieter Riesenberg,Horn Uwe,Knuepfer Uwe,Kujau Marian,Rolf Wenderoth,Andreas Plückthun,Anke Krebber +8 more
TL;DR: In this article, a fed-batch fermentation process using special E. coli host/vector systems for the purpose of efficiently forming recombinant proteins, in particular recombinant antibody molecules, preferably antibody fragments such as miniantibodies.
Journal ArticleDOI
An online monitoring system based on a synthetic sigma32-dependent tandem promoter for visualization of insoluble proteins in the cytoplasm of Escherichia coli.
TL;DR: Data of misfolding studies showed that the ibpfxs::lucA reporter unit provides an adequate tool for in vivo folding studies in E. coli from microtiter up to fermentation scales.
Journal ArticleDOI
A dual expression platform to optimize the soluble production of heterologous proteins in the periplasm of Escherichia coli.
Mario Kraft,Uwe Knüpfer,Rolf Wenderoth,Andre Kacholdt,Patricia Pietschmann,Björn Hock,Uwe Horn +6 more
TL;DR: A dual expression system, containing a degP-promoter-based reporter system and a highly versatile plasmid set, is developed that demonstrates that the amount of functional protein is inversely proportional to the on-line luciferase signal.