Ruijin Yao

TL;DR: Flagellin, which is known to be a glycoprotein, was one of the proteins that showed altered reactivity with O:23 and O:36 antiserum in the mutants, and chemical deglycosylation of protein fractions from the 81‐176 wild type suggests that the other proteins with altered antigenicity in the mutated mutants are also glycosylated.

TL;DR: A method of insertional mutagenesis for naturally transformable organisms has been adapted from Haemophilus influenzae and applied to the study of the pathogenesis of Campylobacter jejuni, which results in the same paralysed flagellar phenotype and the same invasion defects as the original mutants.

TL;DR: A Campylobacter CmR gene cartridge, bracketed by six restriction sites, has been developed for use in site-specific mutagenesis of Campyloblacter genes.

TL;DR: Four motile, non‐adherent and non‐invasive mutants of Campylobacter jejuni 81‐176 generated by a site‐specific insertional mutagenesis scheme were characterized at the molecular level and all contained a duplication of the same region of the chromosome, suggesting that the region contained a repressor of adherence and invasion.

TL;DR: Various bile‐salt supplements were used and it was found that deoxycholate and chenodeoxycholic acid caused a significant enhancement of pilus production and resulted in a highly aggregative phenotype.