Evidence for a system of general protein glycosylation in Campylobacter jejuni.
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TLDR
Flagellin, which is known to be a glycoprotein, was one of the proteins that showed altered reactivity with O:23 and O:36 antiserum in the mutants, and chemical deglycosylation of protein fractions from the 81‐176 wild type suggests that the other proteins with altered antigenicity in the mutated mutants are also glycosylated.Abstract:
A genetic locus from Campylobacter jejuni 81-176 (O:23, 36) has been characterized that appears to be involved in glycosylation of multiple proteins, including flagellin. The lipopolysaccharide (LPS) core of Escherichia coli DH5α containing some of these genes is modified such that it becomes immunoreactive with O:23 and O:36 antisera and loses reactivity with the lectin wheat germ agglutinin (WGA). Site-specific mutation of one of these genes in the E. coli host causes loss of O:23 and O:36 antibody reactivity and restores reactivity with WGA. However, site-specific mutation of each of the seven genes in 81-176 failed to show any detectable changes in LPS. Multiple proteins from various cellular fractions of each mutant showed altered reactivity by Western blot analyses using O:23 and O:36 antisera. The changes in protein antigenicity could be restored in one of the mutants by the presence of the corresponding wild-type allele in trans on a shuttle vector. Flagellin, which is known to be a glycoprotein, was one of the proteins that showed altered reactivity with O:23 and O:36 antiserum in the mutants. Chemical deglycosylation of protein fractions from the 81-176 wild type suggests that the other proteins with altered antigenicity in the mutants are also glycosylated.read more
Citations
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Biological Roles of Glycans
TL;DR: It is time for the diverse functional roles of glycans to be fully incorporated into the mainstream of biological sciences, as they are no different from other major macromolecular building blocks of life, simply more rapidly evolving and complex.
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N-Linked Glycosylation in Campylobacter jejuni and Its Functional Transfer into E. coli
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TL;DR: It is demonstrated that a functional N-linked glycosylation pathway could be transferred into Escherichia coli and opened up the possibility of engineering permutations of recombinant glycan structures for research and industrial applications.
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An evolving view of the eukaryotic oligosaccharyltransferase
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Protein glycosylation in bacteria: sweeter than ever.
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References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.
TL;DR: Results demonstrate that the potentially lethal function specified by fragment B of RK2 is not necessary for replication and that at least one trans-acting function is directly involved in RK 2 replication.
Journal ArticleDOI
Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli.
Rongfu Wang,Sidney R. Kushner +1 more
TL;DR: Using the polymerase chain reaction and standard recombinant DNA techniques, a series of new multipurpose low-copy-number plasmids have been constructed, very useful for analyzing genes encoding proteins which are toxic in Escherichia coli in high copy number.
Journal ArticleDOI
Experimental Campylobacter jejuni Infection in Humans
Robert E. Black,Myron M. Levine,Myron M. Levine,Mary Lou Clements,Mary Lou Clements,Timothy P. Hughes,Timothy P. Hughes,Martin J. Blaser,Martin J. Blaser +8 more
TL;DR: Two strains of Campylobacter jejuni ingested by 111 adult volunteers, in doses ranging from 8 x 10(2) to 2x 10(9) organisms, caused diarrheal illnesses that indicates that the pathogenesis of C.Jejuni infection includes tissue inflammation.
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