S. A. Matlin

TL;DR: It is concluded that the decarboxylation of uroporphyrinogen III to coproporphrins III is a stepwise process taking place by a preferred pathway (both in normal and abnormal metabolism); the acetic acid groups are decar boxylated in a sequential clockwise fashion.

TL;DR: Mixtures of porphyrins derived from natural sources can be readily separated by high-pressure liquid chromatography both analytically and on a preparative scale, and this provides not only a rapid qualitative assessment of the components of a mixture, but also a check on the subsequent chromatographic separations.

TL;DR: Quantitative analyses can be carried out by high-performance liquid chromatography, using appropriate internal standards, and excretion patterns in the various types of porphyria can be obtained which may facilitate clinical diagnosis more effectively than the earlier qualitative thin-layer chromatographic methods.

TL;DR: The results are discussed in relation to the conclusions concerning the preferred pathway of degradation of uro'gen-III to coproporphyrinogen-III, which indicate a clockwise sequence of decarboxylation reactions.

TL;DR: Porphyrin 5 b c d was shown to be more slowly incorporated than porphyrins 5 a b d, 5 a c d and 5 a a b c, and the relevance of these findings to an understanding of the porphirin excretion pattern in hepato-erythrocitic porphyria is considered.